Genome Technology Center

Proximity Ligation for Protein Analyses - Multiplexing protein detection on DNA microarrays


Simon Fredriksson
Michael Mindrinos
Hanlee Ji
Albert Koong
Ronald W. Davis

This project aims to develop a highly scalable multiplexed analysis-platform for proteins. Specific proteins will be converted into unique nucleic acid sequences, molecular barcodes, by homogenous proximity ligation. The resulting pool of protein complement DNA can then be analyzed by traditional methods of molecular genetics such as qPCR and DNA microarrays for relative or absolute measurements.

The protein to DNA transformation is enabled by oligonucleotide tagged antibodies, so called proximity probes, binding pair wise to specific proteins. proximity ligation

They thereby come in such close proximity enabling an enzymatic ligation reaction forming PCR amplicons.




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