Building technology to monitor alternative splicing on a genomic scale

			Personnel Kara Juneau Curtis Palm Ron Davis Related Info Arabidopsis sequencing

This project investigates mRNA splicing patterns that differ in a systematic way between similar cells living in different environments. De-convoluting cellular splicing patterns will lead to a better understanding of the mechanism of pre-mRNA splicing and the factors and sequences that regulate splicing.

Preliminary results from customized real-time PCR protocols on samples from human cell lines and tissue specific cDNAs are promising. Additionally, specialized Arabidopsis specific DNA arrays will be employed to study how different environmental conditions effect splicing patterns in Arabidopsis. The utilization of arrays is expected to expand the number of genes that can be monitored simultaneously.