Genome Technology Center

Nanobiotechnology Projects

Research Projects




PI: Prof. Ronald W. Davis
Rahim Esfandyarpour


Prof. Fabian Pease
Prof. Kenneth Goodson
Prof. Laurent Giovangrandi
Prof. Juan Santiago
Prof. Yoshio Nishi
Prof. Nicholas Melosh
Dr. Jessica Melin
Kosar B. Parizi
Ali Mani

Former Members

Hesaam Esfandyarpour
Melike Abacioglu
Akintunde Maiyegun
Bo Zheng
Neha Ahuja
Dorothy Pan
Elizabeth Burstein
Nancy Dougherty
Elaine Zelby
Eric Lee

Thermosequencing: A Novel Method of DNA sequencing

“Never has the state of DNA sequencing technology been in greater flux than today.”
Michael L. Metzker, Nature Reviews, Genetics 2008.

For the past three decades, Sanger’s method has been the primary DNA sequencing technology; however, inherent limitations in cost and complexity have limited its usage in personalized medicine and ecological studies. Some other methods such as Pyrosequencing or Solexa technology continue to reduce costs and increase throughput; however still the current technologies are not match with the need of individual genome sequencing enabling personalized medicine. [more]


Impedance biosensors are a class of electrical biosensors that show promise for point-of-care and other applications due to low cost, ease of miniaturization and label-free operation. Our Nanoneedle Biosensor as an ultra sensitive and localized biosensor shows a promise to overcome the current limitations of biosensors. This sensor can be used in characterizing biochemical species such as antibodies/antigens or a particular DNA sequence. The system is optimized for the high sensitivity and low concentration detection.

GCMB Microfluidic Biosensor platform

A novel microfluidic platform for DNA sequencing-by-synthesis methods (e.g. pyrosequencing, thermosequencing..). The proposed platform is based on the valve-controllable PDMS channel technology with DNA-coated magnetic beads. The encapsulation of the reaction of DNA polymerization in picoliter-sized wells provides for excellent isolation and control for detection. This separation prevents cross talk amongst neighbor reactors, which is one of the most limitations for higher integration of the current technologies. The proposed system is useful for a number of other bio-species detection and sorting templates.

For further information about these projects please contact:

Rahim Esfandyarpour
E-mail: rahimes at stanford dot edu
Phone: +1-650-812-2745
Fax: +1-650-812-1975

Mailing address:
Stanford Genome Technology Center
855 S. California Avenue
CA 94304

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