Genome Technology Center

Solid-State Nanopore Force Spectroscopy

Vincent Tabard-Cossa
Department of Physics and Astronomy, University of British Columbia, Vancouver, B.C.,Canada

Abstract:
A major barrier to utilization of genetic information in personalized health care is the complexity and time required by existing genotyping processes. Nanopores are emerging as an important platform for the detection and analysis of biomolecules, and may lead to technologies amenable to rapid clinical genotyping.

Our lab is interested in developing nanopore-based force spectroscopy (FS) instruments for rapid characterization of biomolecular interactions. Nanopore FS employs an electric field inside the pore to apply controlled forces to a charged molecule. I will present recent results on receptor-ligand interactions, protein folding/unfolding as well as introduce our genotyping scheme. Single nucleotide polymorphism detection rely on the measurement of the characteristic dissociation time of a DNA duplex under force to provide information on the dissociation energy of the duplex. This, in turn, is an indicator of sequence homology between a single stranded DNA probe of known sequence and a single stranded DNA target.

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