Genome Technology Center

Technologies and Tools for Next Generation Sequencing

Jay Shendure, MD., PhD.

Abstract

We have developed a novel technology for highly multiplexed DNA sequencing based on polony bead amplification and a ligation-based sequencing biochemistry. Proof-of-concept experiments have demonstrated high consensus accuracies and cost-competitiveness with conventional sequencing. Commercial instruments based on this platform or implementing similar concepts are poised to drop the cost of DNA sequencing by two orders of magnitude. To complement these technologies, we are developing methods for multiplex targeted amplification of large sets of human exons from genomic DNA. Partnered with new sequencing technologies, we hope that these methods will enable the highly cost-effective sequencing of the coding subset of human genomes, with applications in cancer genomics and medical resequencing.

 

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