“RNA Structure Probing Using Nanopore Sequencing.”
Jun 26, 2019 (Wed) | 3:00 PM -4:00 PM
MSOB Building Room X303 1265 Welch Rd, Palo Alto, CA 94305
: Stanford, CA
The ability to correctly assign structure information to an individual transcript in a continuous and phased manner is critical to understanding RNA function. RNA structure play important roles in every step of an RNA’s lifecycle, however current short-read high throughput RNA structure mapping strategies are long, complex and cannot assign unique structures to individual gene-linked isoforms in shared sequences. To address these limitations, we present an approach that combines structure probing with nanopore direct RNA sequencing, and machine learning to detect secondary structures on near full-length RNAs (PORE-cupine). PORE-cupine provides rapid, direct, accurate and robust structure information along known RNAs and recapitulates global structural structural features in human embryoni
Department: Dermatology
Contact: daniel braslavsky | 650-725-7022 | danielb4@stanford.edu
Presenter(s):
- Yue Wan Senior Research Scientist, Genome Institute of Singapore
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