CCSB Seminar Series: Single Cell Analysis of Cell Fate Decisions

Jan 23, 2015 (Fri) | 11:00 AM -12:00 PM
Li Ka Shing Center, Room 130 : Stanford, CA

It remains an open question when and how the first cell fate decision is made in mammals. Using deep single-cell RNA-seq of matched sister blastomeres, we report highly reproducible inter-blastomere differences among 10 2-cell and five 4-cell mouse embryos. Inter-blastomere gene expression differences dominated between-embryo differences and noise, and were sufficient to cluster sister blastomeres into distinct groups. Dozens of protein-coding genes exhibited reproducible bimodal expression in sister blastomeres, which cannot be explained by random fluctuations. The highly correlated gene pairs at the 4-cell stage overlapped with those showing the same directions of differential expression between inner cell mass (ICM) and trophectoderm (TE). These data substantiate the hypothesi

Department:  Radiology

Contact: Fuad Nijim | 650-725-6070 | fuad.nijim@stanford.edu

Presenter(s):

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