Blake Meyers,"Phased Small RNAs in Plants: Novel Roles for Secondary siRNAs"
Apr 28, 2014 (Mon) | 4:00 PM -6:00 PM
393 Serra Mall, Herrin T-175 : Stanford, CA
Non-coding RNAs, especially small RNAs, play important roles in many biological processes. Plant small RNA types including microRNAs (miRNAs) and small interfering RNAs (siRNAs), as well as secondary siRNAs that include trans-acting siRNAs (tasiRNAs). Many small RNAs along with their targets have been characterized with deep sequencing technologies, but much remains to be learned about their function. My lab characterizes plant small RNAs and cleaved mRNAs (miRNA targets) in wide variety of species using a suite of custom-built computational tools. The application of these tools to a range of plant species and mutants has identified new miRNAs and targets, as well as diverse populations of phased, secondary siRNAs and their miRNA triggers. The roles in plant gene and genome regulation of these phased siRNAs are poorly characterized, particularly in grass anthers, in which they are extremely abundant, produced by genomically dispersed, long non-coding RNAs (lncRNAs) and synthesized either (1) pre-meiotically during cell fate specification and cell proliferation, or (2) coordinate with germinal cell maturation for meiosis, peak during meiosis, and persisting into mature pollen. In some dicots, such as soybean, hundreds of loci generate phased siRNAs, mainly from protein-coding genes. We aim to understand the regulatory function of these secondary siRNAs from both coding and non-coding RNAs.
Contact: Maria Magana-Lopez | 650-723-2413 | email@example.com