SCIT Program Seminars
The SCIT program hosts a quarterly colloquium during which two trainees present the status of their research.
Wednesday, October 14, 2015
Glazer Learning Center (Lucas, P083)
"Automated Grading of Gliomas in Digital Pathology Images"
Mehmet Ertosun, PhD
Abstract: Brain glioma is the most common primary malignant brain tumors in adults with different pathologic subtypes: Lower Grade Glioma (LGG)and Glioblastoma Multiforme (GBM). The survival and treatment options are highly dependent of the glioma grade. We propose a classification pipeline for automated grading of gliomas using digital pathology images.
"Immune-Modulating Effects of the FDA Approved Iron Oxide Nanoparticle Ferumoxytol Inhibit Tumor Growth"
Saeid Zanganeh, PhD
Abstract: Ferumoxytol is an FDA-approved superparamagnetic iron oxide nanoparticle (USPIO) used for diagnostic in vivo imaging. Ferumoxytol is preferentially phagocytosed by tumor-associated macrophages (TAMs), and enables quantitative evaluation of initial tumor perfusion, tumor retention and persistent magnetic resonance-enhancement. Surprisingly, mammary adenocarcinoma-bearing mice injected with ferumoxytol had significantly smaller tumors at end-stage as compared to littermate controls injected with PBS. To determine mechanisms underlying ferumoxytol-induced tumor suppression, we examined the impact of ferumoxytol on macrophages and malignant tumor cells using in vitro and in vivo assays. Our data revealed no induced cytotoxicity to mammary adenocarcinoma cells incubated with either ferumoxytol or macrophages; however, when added in combination, carcinoma cells exhibited increased cleaved caspase-3 positivity. Macrophages exposed to ferumoxytol in vitro exhibited altered gene expression programing wherein mRNAs associated with pro-inflammatory TH1-type responses were increased, e.g., TNFa, iNOS,CD68, and mRNAs associated with immune-suppression and TH2-type responses instead decreased, e.g.,IL10, CD206. In vivo, mammary adenocarcinoma growth was significantly suppressed in mice injected with intratumoral ferumoxytol, and correlated with increased presence of monocyte/macrophages exhibiting an immune-stimulatory or TH1-type phenotype as revealed by histopathology and FACS.