Dehua (Eddy) Zhao, Ph.D.


1999 - 2004

Ph.D. in Microbiology

China Agricultural University, Beijing, China

1995 - 1999

B.S. in Microbiology

China Agricultural University, Beijing, China



Xiong X, Chen M, Lim WA, Zhao D, Qi LS. CRISPR/Cas9 for Human Genome Engineering and Disease Research. Annu. Rev. Genomics Hum. Genet. 2016 May 23    

Peters JM, Silvis MR, Zhao D, Hawkins JS, Gross CA, Qi LS. Bacterial CRISPR: accomplishments and prospects. Curr. Opin. Microbiol. 27: 121–126. 2015 Sep 9.    

Smanski MJ, Bhatia S, Zhao D, Park Y, B A Woodruff L, Giannoukos G, Ciulla D, Busby M, Calderon J, Nicol R, Gordon DB, Densmore D, Voigt CA. Functional optimization of gene clusters by combinatorial design and assembly. Nat. Biotechnol. 32, 1241-1249 (2014). 

Wang L, Zhang L, Liu Z, Zhao D, Liu X, Zhang B, Xie J, Hong Y, Li P, Chen S, Dixon R, Li J. A Minimal Nitrogen Fixation Gene Cluster from Paenibacillus sp. WLY78 Enables Expression of Active Nitrogenase in Escherichia coli. PLoS Genet. 9, e1003865 (2013).

Temme K*, Zhao D*, Voigt CA. Refactoring the nitrogen fixation gene cluster from Klebsiella oxytoca. Proc. Natl. Acad. Sci. U.S.A109, 7085-7090 (2012). (*co-first author).

Hernandez JA, Phillips AH, Erbil WK, Zhao D, Demuez M, Zeymer C, Pelton JG, Wemmer DE, Rubio LM. A sterile alpha motif domain in NafY targets apo-nitrogenase for FeMo-cofactor delivery via a tethered domain. J. Biol. Chem. 286, 6321-6328 (2011).

Soboh B, Boyd ES, Zhao D, Peters JW, and Rubio LM. Substrate specificity and evolutionary implications of a NifDK enzyme carrying NifB-co at its active site. FEBS Lett 584, 1487-1492 (2010).

George SJ, Igarashi RY, Xiao Y, Hernandez JA, Demuez M, Zhao D, Yoda Y, Ludden PW, Rubio LM, Cramer SP. Extended X-ray absorption fine structure and nuclear resonance vibrational spectroscopy reveal that NifB-co, a FeMo-co precursor, comprises a 6Fe core with an interstitial light atom. J Am Chem Soc.130, 5673-5680 (2008).

Zhao D, Curatti L, Rubio LM. Evidence for NifU and NifS participation in the biosynthesis of the iron-molybdenum cofactor of nitrogenase. J Biol Chem.282, 37016-37025 (2007).

Curatti L, Hernandez JA, Igarashi RY, Soboh B, Zhao D, Rubio LM. In vitro synthesis of the iron-molybdenum cofactor of nitrogenase from iron, sulfur, molybdenum, and homocitrate using purified proteins. Proc. Natl. Acad. Sci. U.S.A. 104, 17626-17631 (2007).

Guan F, Zhao D, Pan M, Jiang W, Li J. Analysis of active sites for N2 and H+ reduction on the FeMo-cofactor of nitrogenase. Chinese Science Bulletin 52, 2088-2094 (2007).

Zhao D, Li J. Construction and Characterization of double mutants in nitrogenase of Klebsiella pneumoniae. Chinese Science Bulletin 48, 1-7 (2004).

Jiang W, Zhao D, Li Y, Tian J, Wang Z, Li J. Submerged culture of Magnetospirillum gryphiswaldense under N2-fixing condition and regulation of activity of nitrogen fixation. Chinese Science Bulletin 47, 2095-2099 (2002).