Elisabetta Viani Puglisi
Academic Appointments
- Assistant Professor (Research), Structural Biology
Contact Information
- Academic
Offices
Personal Information Email Tel (650) 498-5420
Professional Snapshot
Honors and Awards
- Graduate Fellowship, Italian Ministry of Education and Scientific Research (1991-1993)
- Fellowhip for University Research, Italian Pharmacy Consurtium (1989-1990)
- Fellowship for University Research, European Economic Community (1988)
Professional Education
| Ph.D.: | University of Brescia, Italy, Microbiology (1994) |
| Dott.: | University of Parma, Italy, Chemistry (1989) |
| Laurea: | University of Parma, Italy, Chemistry (1985) |
Scientific Focus
Research Interests
Viral infections and subsequent host response depend on multiple RNA-protein interaction. My research focuses on the structural and functional characterization of RNA-protein complexes involved in viral infection. Current research aims to understand how the Human Immunodeficiency Virus (HIV) initiates its replication upon host infection. We use NMR spectroscopy and x-ray crystallography to study the structure of the initiation complex, formed by a host tRNA and HIV genomic RNA, coupled with biochemical and biophysical methods to understand functional properties. The goal of this research is to gain a molecular view of HIV replication initiation, and use this information to develop new therapeutic approaches to combat HIV.
Publications
- Probing the conformation of human tRNA(3)(Lys) in solution by NMR. FEBS Lett. 2007; (27): 5307-14
- Rapid purification of RNAs using fast performance liquid chromatography (FPLC). RNA. 2007; (2): 289-94
- Purification and characterization of transcribed RNAs using gel filtration chromatography. Nat Protoc. 2007; (12): 3270-7
- HIV-1 A-rich RNA loop mimics the tRNA anticodon structure. Nat Struct Biol. 1998; (12): 1033-6
- Structure of a conserved RNA component of the peptidyl transferase centre. Nat Struct Biol. 1997; (10): 775-8
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