Sanjiv Sam Gambhir, MD, PhD

Publication Details

• Rapid synthesis of a 5'-fluorinated oligodeoxy-nucleotide: a model antisense probe for use in imaging with positron emission tomography (PET).

  Pan D, Gambhir SS, Toyokuni T, Iyer MR, Acharya N, Phelps ME, Barrio JR. Bioorg Med Chem Lett. 1998; 8 (11): 1317-20

  5'-Deoxy-5'-fluoro-O4-methylthymidine was synthesized by the reaction of the corresponding 5'-O-tosylate with KF in the presence of Kryptofix [222] and coupled to a 5'-phosphoramidite-activated CPG-bound oligodeoxynucleotide. The sequence of reactions and purifications were accomplished within 4 h, a necessary condition of the development of radiofluorinated antisense oligodeoxynucleotide probe for use with PET.

  PubMedID: 9871758