Brian Hoffman
Publication Details
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Effects of cell density and conditioned medium on alpha 2A adrenergic receptor density and messenger RNA abundance in HT-29 cells.
Life Sci. 1994; (23): 1785-91
We have examined the relationship between cell density and alpha 2A adrenergic receptor number and mRNA in HT-29 cells. alpha 2A receptors increased with increasing cell density from 75 +/- 25 to 400 +/- 73 fmol/mg protein; alpha 2A receptor mRNA also increased about 4 fold with no change in abundance of beta-actin mRNA. We prepared serum-free conditioned medium (SFCM) from confluent dishes of HT-29 cells and applied this SFCM to cells seeded at low density. A 4.7-fold increase in receptor number was observed in HT-29 cells cultured in SFCM for 3 days and alpha 2A receptor mRNA level increased about 3-fold after 24 h. Dialysis of SFCM against fresh growth medium did not abolish these effects. These results suggest that a substance secreted from HT-29 cells regulates expression of alpha 2A receptors.
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