Sanjiv Sam Gambhir, MD, PhD
Publication Details
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Noninvasive monitoring of placenta-specific transgene expression by bioluminescence imaging.
PLoS One. 2011; (1): e16348
Placental dysfunction underlies numerous complications of pregnancy. A major obstacle to understanding the roles of potential mediators of placental pathology has been the absence of suitable methods for tissue-specific gene manipulation and sensitive assays for studying gene functions in the placentas of intact animals. We describe a sensitive and noninvasive method of repetitively tracking placenta-specific gene expression throughout pregnancy using lentivirus-mediated transduction of optical reporter genes in mouse blastocysts.
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