Garry Nolan
Publication Details
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Intracellular phospho-protein staining techniques for flow cytometry: monitoring single cell signaling events.
Cytometry A. 2003; (2): 61-70
Recent advances in intracellular staining techniques, cytometer technology, fluorescent reagents, and antibody production have expanded the number of intracellular antigens that can be analyzed by flow cytometry. Measurement of protein phosphorylation with phospho-specific antibodies has given insight into kinase signaling cascades. However, available techniques for phospho-epitope staining can differ greatly, making it necessary to understand the differences between the outcomes when such techniques are applied and to develop robust and reproducible methods of application.
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