Mark M. Davis
Publication Details
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Simultaneous detection of many T-cell specificities using combinatorial tetramer staining.
Nat Methods. 2009; (7): 497-9The direct detection of antigen-specific T cells using tetramers of soluble peptide-major histocompatibilty complex (pMHC) molecules is widely used in both basic and clinical immunology. However, the number of specificities that can be assessed simultaneously has been a major limitation. Here we describe and validate a method using combinations of fluorescent pMHC tetramers to simultaneously detect and enrich for many (>or=15) T-cell specificities in a single human blood sample.
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