Greg Barsh
Publication Details
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Gene trap insertional mutagenesis in mice: new vectors and germ line mutations in two novel genes.
Transgenic Res. 1999; (6): 451-8
Insertional mutagenesis based on gene trap vectors that capture endogenous splice sites is a promising tool for functional genomics. Several groups have proposed large-scale gene trap screens, but questions remain as to the type of vectors and their design. We report a set of plasmid-encoded gene trap vectors and the disruption of two novel genes. Our results include a comparison of the relative gene trapping efficiencies of two different splice acceptor sequences in ES cells and an analysis of the structure of several gene trap insertions.
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