Giles W Plant
Publication Details
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Embryonic-derived olfactory ensheathing cells remyelinate focal areas of spinal cord demyelination more efficiently than neonatal or adult derived cells.
Cell Transplant. 2012
Transplanted olfactory ensheathing cells (OECs) contribute to functional recovery in a range of CNS injuries by several mechanisms, one of which is potentially their ability to form myelin sheaths. OECs sourced from donors of different ages have been shown to remyelinate in several in vitro and in vivo models. However, the optimal donor age for OEC associated remyelination is unclear. This project directly compared the remyelinating potential of p75 purified OEC transplants from three donor ages. OECs were sourced from the olfactory bulbs of embryonic, neonatal and adult rats, purified by immunopanning and their remyelinating potential directly compared by transplantation into the same adult rat toxin-induced model of spinal cord demyelination. Remyelination efficiency three weeks after transplant was assessed morphologically and by immunostaining. Our results indicate that all donor ages remyelinate, however this process is most efficiently achieved by embryonic-derived OECs.
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