Mark A. Kay, M.D., Ph.D.
Publication Details
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A nonviral minicircle vector for deriving human iPS cells.
Nat Methods. 2010; (3): 197-9
Owing to the risk of insertional mutagenesis, viral transduction has been increasingly replaced by nonviral methods to generate induced pluripotent stem cells (iPSCs). We report the use of 'minicircle' DNA, a vector type that is free of bacterial DNA and capable of high expression in cells, for this purpose. Here we use a single minicircle vector to generate transgene-free iPSCs from adult human adipose stem cells.
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