Stephen Quake

Honors and Awards

• NIH Director's Pioneer Award, NIH (2004)
• 100 Young Innovators that will create the future, MIT Tech Review Magazine (2002)
• Participant, NAS Symposium for Frontiers in Science (1999, 2000)
• Packard Fellow, Packard Foundation (1999)
• Career Award, NSF (1997)
• R29 "FIRST" Award, NIH (1997)

Professional Education

• Ph.D., University of Oxford Physics (1994)
• M.S., Stanford University Mathematics (1991)
• B.S., Stanford University Physics (1991)

Postdoctoral Advisees

• Paul Blainey, Ning Jiang, Tomer Kalisky, Matthias Meier

Graduate & Fellowship Program Affiliations

• Bioengineering

Web Site Links

• Quake Lab Site

Research Interests

Single molecule biophysics, precision force measurement, micro and nano fabrication with soft materials, integrated microfluidics and large scale biological automation.

Publications

• Solvent resistant microfluidic DNA synthesizer. Huang Y,  Castrataro P, Lee CC, Quake SR.  Lab Chip.  2007; 7 (1): 24-6
• Systematic investigation of protein phase behavior with a microfluidic formulator. Hansen CL,  Sommer MO, Quake SR.  Proc Natl Acad Sci U S A.  2004; 101 (40): 14431-6
• Microfluidic device reads up to four consecutive base pairs in DNA sequencing-by-synthesis. Kartalov EP,  Quake SR.  Nucleic Acids Res.  2004; 32 (9): 2873-9
• A microfluidic rectifier: anisotropic flow resistance at low Reynolds numbers. Groisman A,  Quake SR.  Phys Rev Lett.  2004; 92 (9): 094501
• A nanoliter-scale nucleic acid processor with parallel architecture. Hong JW,  Studer V, Hang G, Anderson WF, Quake SR.  Nat Biotechnol.  2004; 22 (4): 435-9
• Solvent-resistant photocurable liquid fluoropolymers for microfluidic device fabrication [corrected]. Rolland JP,  Van Dam RM, Schorzman DA, Quake SR, DeSimone JM.  J Am Chem Soc.  2004; 126 (8): 2322-3
• Tip-enhanced fluorescence microscopy at 10 nanometer resolution. Gerton JM,  Wade LA, Lessard GA, Ma Z, Quake SR.  Phys Rev Lett.  2004; 93 (18): 180801
• Quantifying double-strand breaks and clustered damages in DNA by single-molecule laser fluorescence sizing. Filippova EM,  Monteleone DC, Trunk JG, Sutherland BM, Quake SR, Sutherland JC.  Biophys J.  2003; 84 (2 Pt 1): 1281-90
• Sequence information can be obtained from single DNA molecules. Braslavsky I,  Hebert B, Kartalov E, Quake SR.  Proc Natl Acad Sci U S A.  2003; 100 (7): 3960-4
• Polyelectrolyte surface interface for single-molecule fluorescence studies of DNA polymerase. Kartalov EP,  Unger MA, Quake SR.  Biotechniques.  2003; 34 (3): 505-10
• Microfluidic memory and control devices. Groisman A,  Enzelberger M, Quake SR.  Science.  2003; 300 (5621): 955-8
• Microfabricated fountain pens for high-density DNA arrays. Reese MO,  van Dam RM, Scherer A, Quake SR.  Genome Res.  2003; 13 (10): 2348-52
• Integrated nanoliter systems. Hong JW,  Quake SR.  Nat Biotechnol.  2003; 21 (10): 1179-83
• Microfluidics in structural biology: smaller, faster em leader better. Hansen C,  Quake SR.  Curr Opin Struct Biol.  2003; 13 (5): 538-44
• Solving the "world-to-chip" interface problem with a microfluidic matrix. Liu J,  Hansen C, Quake SR.  Anal Chem.  2003; 75 (18): 4718-23
• Behavior of complex knots in single DNA molecules. Bao XR,  Lee HJ, Quake SR.  Phys Rev Lett.  2003; 91 (26 Pt 1): 265506
• Gene expression analysis with universal n-mer arrays. van Dam RM,  Quake SR.  Genome Res.  2002; 12 (1): 145-52
• An integrated microfabricated cell sorter. Fu AY,  Chou HP, Spence C, Arnold FH, Quake SR.  Anal Chem.  2002; 74 (11): 2451-7
• A nanoliter rotary device for polymerase chain reaction. Liu J,  Enzelberger M, Quake S.  Electrophoresis.  2002; 23 (10): 1531-6
• Velocity-independent microfluidic flow cytometry. Eyal S,  Quake SR.  Electrophoresis.  2002; 23 (16): 2653-7
• Significance and statistical errors in the analysis of DNA microarray data. Brody JP,  Williams BA, Wold BJ, Quake SR.  Proc Natl Acad Sci U S A.  2002; 99 (20): 12975-8
• Identification and confirmation of a module of coexpressed genes. Thompson HG,  Harris JW, Wold BJ, Quake SR, Brody JP.  Genome Res.  2002; 12 (10): 1517-22
• Number, density, and surface/cytoplasmic distribution of GABA transporters at presynaptic structures of knock-in mice carrying GABA transporter subtype 1-green fluorescent protein fusions. Chiu CS,  Jensen K, Sokolova I, Wang D, Li M, Deshpande P, Davidson N, Mody I, Quick MW, Quake SR, Lester HA.  J Neurosci.  2002; 22 (23): 10251-66
• A robust and scalable microfluidic metering method that allows protein crystal growth by free interface diffusion. Hansen CL,  Skordalakes E, Berger JM, Quake SR.  Proc Natl Acad Sci U S A.  2002; 99 (26): 16531-6
• Microfluidic large-scale integration. Thorsen T,  Maerkl SJ, Quake SR.  Science.  2002; 298 (5593): 580-4
• Dynamic pattern formation in a vesicle-generating microfluidic device. Thorsen T,  Roberts RW, Arnold FH, Quake SR.  Phys Rev Lett.  2001; 86 (18): 4163-6
• Single-molecule measurements calibrate green fluorescent protein surface densities on transparent beads for use with 'knock-in' animals and other expression systems. Chiu CS,  Kartalov E, Unger M, Quake S, Lester HA.  J Neurosci Methods.  2001; 105 (1): 55-63
• Monolithic microfabricated valves and pumps by multilayer soft lithography. Unger MA,  Chou HP, Thorsen T, Scherer A, Quake SR.  Science.  2000; 288 (5463): 113-6
• From micro- to nanofabrication with soft materials. Quake SR,  Scherer A.  Science.  2000; 290 (5496): 1536-40
• Femtonewton force spectroscopy of single extended DNA molecules. Meiners JC,  Quake SR.  Phys Rev Lett.  2000; 84 (21): 5014-7
• Single-molecule fluorescence observed with mercury lamp illumination. Unger M,  Kartalov E, Chiu CS, Lester HA, Quake SR.  Biotechniques.  1999; 27 (5): 1008-14
• A microfabricated fluorescence-activated cell sorter. Fu AY,  Spence C, Scherer A, Arnold FH, Quake SR.  Nat Biotechnol.  1999; 17 (11): 1109-11
• A microfabricated device for sizing and sorting DNA molecules. Chou HP,  Spence C, Scherer A, Quake S.  Proc Natl Acad Sci U S A.  1999; 96 (1): 11-3
• The dynamics of partially extended single molecules of DNA. Quake SR,  Babcock H, Chu S.  Nature.  1997; 388 (6638): 151-4
• Fast Monte Carlo algorithms for knotted polymers. Quake SR,  Phys Rev E Stat Phys Plasmas Fluids Relat Interdiscip Topics.  1995; 52 (1): 1176-1180
• Relaxation of a single DNA molecule observed by optical microscopy. Perkins TT,  Quake SR, Smith DE, Chu S.  Science.  1994; 264 (5160): 822-6
• Topological effects of knots in polymers. Quake SR,  Phys Rev Lett.  1994; 73 (24): 3317-3320