Manuel Amieva

Clinical Focus

• Infectious Diseases, Pediatric
• Pediatric Infectious Disease

Professional Education

• Board Certification: Pediatric Infectious Disease, (2005)
• Stanford University School of Medicine (2004) CA
• Board Certification: Pediatrics, American Board of Pediatrics (2003)
• Stanford University School of Medicine (1999) CA
• Stanford University School of Medicine (1998) CA
• Stanford University School of Medicine (1997) CA

Postdoctoral Advisees

• Mickey Pentecost, Lee Shaughnessy

Graduate & Fellowship Program Affiliations

• Microbiology and Immunology
• Pediatrics

Research Interests

My laboratory studies the strategies pathogens utilize to colonize and subvert the epithelial barrier. We have focused on the epithelial junctions as a target for bacterial pathogens, since the cell-cell junctions serve as both a barrier to infection and also a major control site for epithelial function. In particular, we are interested in how the gastric pathogen Helicobater pylori may cause cancer by interfering with cell signaling at the epithelial junctions. We are also studying how various bacteria cross and invade the epithelium. For example, we recently found that Listeria monocytogenes targets a specialized subset of cell-cell junctions at the tip of the intestinal villi to find its receptor for invasion. We are interested in determining whether this mode of gastrointestinal invasion of the epithelium is also used by other gastrointestinal pathogens.

Publications

• The complete genome sequence of Helicobacter pylori strain G27. Baltrus DA,  Amieva MR, Covacci A, Lowe TM, Merrell DS, Ottemann KM, Stein M, Salama NR, Guillemin K.  J Bacteriol.  2009; 191 (1): 447-8
• Host-bacterial interactions in Helicobacter pylori infection. Amieva MR,  El-Omar EM.  Gastroenterology.  2008; 134 (1): 306-23
• Listeria monocytogenes invades the epithelial junctions at sites of cell extrusion. Pentecost M,  Otto G, Theriot JA, Amieva MR.  PLoS Pathog.  2006; 2 (1): e3
• Helicobacter pylori CagA induces a transition from polarized to invasive phenotypes in MDCK cells. Bagnoli F,  Buti L, Tompkins L, Covacci A, Amieva MR.  Proc Natl Acad Sci U S A.  2005; 102 (45): 16339-44
• Helicobacter pylori and gastric cancer: what can be learned by studying the response of gastric epithelial cells to the infection? Mueller A,  Falkow S, Amieva MR.  Cancer Epidemiol Biomarkers Prev.  2005; 14 (8): 1859-64
• Important bacterial gastrointestinal pathogens in children: a pathogenesis perspective. Amieva MR,  Pediatr Clin North Am.  2005; 52 (3): 749-77, vi
• Breaking into the epithelial apical-junctional complex--news from pathogen hackers. Vogelmann R,  Amieva MR, Falkow S, Nelson WJ.  Curr Opin Cell Biol.  2004; 16 (1): 86-93
• Disruption of the epithelial apical-junctional complex by Helicobacter pylori CagA. Amieva MR,  Vogelmann R, Covacci A, Tompkins LS, Nelson WJ, Falkow S.  Science.  2003; 300 (5624): 1430-4
• Free latissimus dorsi flap used in treatment of cerebral aspergillosis: a case report and review of the literature. Antony AK,  Hong RW, Amieva M, Taekman MS, Huhn SL, Karanas YL.  Microsurgery.  2003; 23 (4): 313-6
• Helicobacter pylori enter and survive within multivesicular vacuoles of epithelial cells. Amieva MR,  Salama NR, Tompkins LS, Falkow S.  Cell Microbiol.  2002; 4 (10): 677-90
• Jarisch-Herxheimer reaction associated with ciprofloxacin administration for tick-borne relapsing fever. Webster G,  Schiffman JD, Dosanjh AS, Amieva MR, Gans HA, Sectish TC.  Pediatr Infect Dis J.  2002; 21 (6): 571-3
• Imaging of dynamic changes of the actin cytoskeleton in microextensions of live NIH3T3 cells with a GFP fusion of the F-actin binding domain of moesin. Litman P,  Amieva MR, Furthmayr H.  BMC Cell Biol.  2000; 1 1
• Disruption of dynamic cell surface architecture of NIH3T3 fibroblasts by the N-terminal domains of moesin and ezrin: in vivo imaging with GFP fusion proteins. Amieva MR,  Litman P, Huang L, Ichimaru E, Furthmayr H.  J Cell Sci.  1999; 112 ( Pt 1) 111-25
• The plasma membrane-actin linking protein, ezrin, is a glomerular epithelial cell marker in glomerulogenesis, in the adult kidney and in glomerular injury. Hugo C,  Nangaku M, Shankland SJ, Pichler R, Gordon K, Amieva MR, Couser WG, Furthmayr H, Johnson RJ.  Kidney Int.  1998; 54 (6): 1934-44
• Hypoxia increases human keratinocyte motility on connective tissue. O'Toole EA,  Marinkovich MP, Peavey CL, Amieva MR, Furthmayr H, Mustoe TA, Woodley DT.  J Clin Invest.  1997; 100 (11): 2881-91
• Phosphorylation of 558T of moesin detected by site-specific antibodies in RAW264.7 macrophages. Nakamura F,  Amieva MR, Hirota C, Mizuno Y, Furthmayr H.  Biochem Biophys Res Commun.  1996; 226 (3): 650-6
• The cytoskeletal linking proteins, moesin and radixin, are upregulated by platelet-derived growth factor, but not basic fibroblast growth factor in experimental mesangial proliferative glomerulonephritis. Hugo C,  Hugo C, Pichler R, Gordon K, Schmidt R, Amieva M, Couser WG, Furthmayr H, Johnson RJ.  J Clin Invest.  1996; 97 (11): 2499-508
• Subcellular localization of moesin in dynamic filopodia, retraction fibers, and other structures involved in substrate exploration, attachment, and cell-cell contacts. Amieva MR,  Furthmayr H.  Exp Cell Res.  1995; 219 (1): 180-96
• Phosphorylation of threonine 558 in the carboxyl-terminal actin-binding domain of moesin by thrombin activation of human platelets. Nakamura F,  Amieva MR, Furthmayr H.  J Biol Chem.  1995; 270 (52): 31377-85
• Moesin, ezrin, and p205 are actin-binding proteins associated with neutrophil plasma membranes. Pestonjamasp K,  Amieva MR, Strassel CP, Nauseef WM, Furthmayr H, Luna EJ.  Mol Biol Cell.  1995; 6 (3): 247-59
• Radixin is a component of hepatocyte microvilli in situ. Amieva MR,  Wilgenbus KK, Furthmayr H.  Exp Cell Res.  1994; 210 (1): 140-4
• Moesin, a new cytoskeletal protein and constituent of filopodia: its role in cellular functions. Furthmayr H,  Lankes W, Amieva M.  Kidney Int.  1992; 41 (3): 665-70
• Early neurogenesis of the mouse olfactory nerve: Golgi and electron microscopic studies. Marin-Padilla M,  Amieva MR.  J Comp Neurol.  1989; 288 (2): 339-52