James Spudich

Administrative Appointments

• Co-Founder and first Director, Interdisciplinary Program, Bio-X, Stanford University , (1998– 2002 )
• Co-Founder, Cytokinetics, Inc , (1998– present )
• Chairman, Department of Biochemistry, Stanford University School of Medicine , (1994– 1998 )
• Professor, Department of Biochemistry, Stanford University School of Medicine , (1992– present )
• Professor, Department of Developmental Biology, Stanford University School of Medicine , (1989– present )
• Chairman, Department of Structural Biology, Stanford University School of Medicine , (1979– 1984 )
• Professor, Department of Structural Biology, Stanford University School of Medicine , (1977– 1992 )
• Professor, Department of Biochemistry and Biophysics, University of California, San Francisco , (1976– 1977 )
• Associate Professor, Department of Biochemistry & Biophysics, University of California, San Francisco , (1974– 1976 )
• Assistant Professor, Department of Biochemistry & Biophysics, University of California, San Francisco , (1971– 1974 )

Honors and Awards

• U.S. Genomics Award for Outstanding Investigator in the field of Single Molecule Biology, Biophysical Society (2006)
• Named the "Douglass M. and Nola Leishman Professor of Cardiovascular Disease", Stanford University (1987 - present)
• Elected Member of the National Academy of Sciences, the National Academy of Sciences (1991)
• American Heart Association Research Prize, National American Heart Association (1991)
• Alexander von Humboldt Research Award, Alexander von Humboldt Research Foundation (1991)
• NIH Merit Award, National Institutes of Health (1991)
• External Scientific Member of the Max-Planck-Institute für Biochemie in Martinsried bei München, Max-Planck Society (1994)
• Biophysical Society Lifetime Research Career Award, Biophysical Society (1995)
• Lewis S. Rosenstiel Award, Brandeis University (1996)
• 1997 Repligen Award in Chemistry of Biological Processes, Division of Biological Chemistry of the American Chemical Society (1996)
• Elected Fellow of the American Academy of Arts and Sciences, the American Academy of Arts and Sciences (1997)
• Elected Fellow of the American Association for the Advancement of Science, the American Association for the Advancement of Science (2001)

Professional Education

• B.S., University of Illinois Chemistry (1963)
• Ph.D., Stanford University Biochemistry (1968)
• Postdoctoral, Stanford University Genetics (1969)
• Postdoctoral, Cambridge University, MRC LMB Structural Biology (1971)

Postdoctoral Advisees

• Sivaraj Sivaramakrishnan, Roopa Taranath

Industry Relationships

Stanford is committed to ethical and transparent interactions with our industry partners. It is our policy to disclose payments of $5,000 or more, equity valued at $5,000 or more in a publicly traded company, or any equity in a privately held company, to physicians and scientists employed by Stanford University from companies or other commercial entities with which they interact as part of their professional activities. 

• Consulting: Cytokinetics, Inc.
• Equity: Cytokinetics, Inc.
• Service on Board of Directors: Cytokinetics, Inc.

Research Interests

The general research interest of this laboratory is the molecular basis of cell motility. We have three specific research interests, the molecular basis of energy transduction that leads to ATP-driven myosin movement on actin, the biochemical basis of the regulation of actin and myosin interaction and their assembly states, and the roles these proteins play in vivo, in cell movement and changes in cell shape.

We work on two experimental systems: contraction of mammalian muscle and chemotaxis of Dictyostelium discoideum cells. Each of these systems has its special advantages. Skeletal muscle has the most highly organized contractile apparatus of any cell type, and the chemistry and biochemistry of muscle actin and myosin are most advanced.

Dictyostelium discoideum, the cell that commands most of our attention, exhibits all of the behavior of nonmuscle mammalian cells and, unlike other eukaryotic cells, can be grown in large amounts for biochemical work. Furthermore, DNA-mediated transformation is being applied to this organism, and we have demonstrated efficient gene targeting by homologous recombination in the myosin gene, which we have cloned and sequenced.

Our approaches include biochemical and structural studies of actin, myosin, and associated regulatory proteins. In addition, we have designed and developed in vitro assays for ATP-dependent movement of purified myosin on filaments reconstituted from purified actin. These assays allow us to analyze mutant myosin molecules for altered function. The site-directed mutagenized forms of myosin are obtained by gene cloning and expression in an appropriate host. Our demonstration that the Dictyostelium discoideum myosin gene can undergo homologous recombination allows us to also probe the effects of the altered myosin forms on the phenotype of the cell.

Publications

• Engineered myosin VI motors reveal minimal structural determinants of directionality and processivity. Liao JC,  Elting MW, Delp SL, Spudich JA, Bryant Z.  J Mol Biol.  2009; 392 (4): 862-7
• Dynamic organization of gene loci and transcription compartments in the cell nucleus. Spudich JA,  Biophys J.  2008; 95 (11): 5003-4
• Long single alpha-helical tail domains bridge the gap between structure and function of myosin VI. Spink BJ,  Sivaramakrishnan S, Lipfert J, Doniach S, Spudich JA.  Nat Struct Mol Biol.  2008; 15 (6): 591-7
• Molecular motors: a surprising twist in myosin VI translocation. Spudich JA,  Curr Biol.  2008; 18 (2): R68-70
• Dynamics of the unbound head during myosin V processive translocation. Dunn AR,  Spudich JA.  Nat Struct Mol Biol.  2007; 14 (3): 246-8
• Precise positioning of myosin VI on endocytic vesicles in vivo. Altman D,  Goswami D, Hasson T, Spudich JA, Mayor S.  PLoS Biol.  2007; 5 (8): e210
• Predicting allosteric communication in myosin via a pathway of conserved residues. Tang S,  Liao JC, Dunn AR, Altman RB, Spudich JA, Schmidt JP.  J Mol Biol.  2007; 373 (5): 1361-73
• The localization of inner centromeric protein (INCENP) at the cleavage furrow is dependent on Kif12 and involves interactions of the N terminus of INCENP with the actin cytoskeleton. Chen Q,  Lakshmikanth GS, Spudich JA, De Lozanne A.  Mol Biol Cell.  2007; 18 (9): 3366-74
• Identification of a minimal myosin Va binding site within an intrinsically unstructured domain of melanophilin. Geething NC,  Spudich JA.  J Biol Chem.  2007; 282 (29): 21518-28
• Extending the absorbing boundary method to fit dwell-time distributions of molecular motors with complex kinetic pathways. Liao JC,  Spudich JA, Parker D, Delp SL.  Proc Natl Acad Sci U S A.  2007; 104 (9): 3171-6
• The power stroke of myosin VI and the basis of reverse directionality. Bryant Z,  Altman D, Spudich JA.  Proc Natl Acad Sci U S A.  2007; 104 (3): 772-7
• Rho kinase's role in myosin recruitment to the equatorial cortex of mitotic Drosophila S2 cells is for myosin regulatory light chain phosphorylation. Dean SO,  Spudich JA.  PLoS One.  2006; 1 e131
• Molecular motors take tension in stride. Spudich JA,  Cell.  2006; 126 (2): 242-4
• A non-Gaussian distribution quantifies distances measured with fluorescence localization techniques. Churchman LS,  Flyvbjerg H, Spudich JA.  Biophys J.  2006; 90 (2): 668-71
• A force-dependent state controls the coordination of processive myosin V. Purcell TJ,  Sweeney HL, Spudich JA.  Proc Natl Acad Sci U S A.  2005; 102 (39): 13873-8
• A flexible domain is essential for the large step size and processivity of myosin VI. Rock RS,  Ramamurthy B, Dunn AR, Beccafico S, Rami BR, Morris C, Spink BJ, Franzini-Armstrong C, Spudich JA, Sweeney HL.  Mol Cell.  2005; 17 (4): 603-9
• Distinct pathways control recruitment and maintenance of myosin II at the cleavage furrow during cytokinesis. Dean SO,  Rogers SL, Stuurman N, Vale RD, Spudich JA.  Proc Natl Acad Sci U S A.  2005; 102 (38): 13473-8
• Single molecule high-resolution colocalization of Cy3 and Cy5 attached to macromolecules measures intramolecular distances through time. Churchman LS,  Okten Z, Rock RS, Dawson JF, Spudich JA.  Proc Natl Acad Sci U S A.  2005; 102 (5): 1419-23
• Myosin VI walks hand-over-hand along actin. Okten Z,  Churchman LS, Rock RS, Spudich JA.  Nat Struct Mol Biol.  2004; 11 (9): 884-7
• The mechanism of myosin VI translocation and its load-induced anchoring. Altman D,  Sweeney HL, Spudich JA.  Cell.  2004; 116 (5): 737-49
• A mitotic kinesin-like protein required for normal karyokinesis, myosin localization to the furrow, and cytokinesis in Dictyostelium. Lakshmikanth GS,  Warrick HM, Spudich JA.  Proc Natl Acad Sci U S A.  2004; 101 (47): 16519-24
• Two important polymers cross paths. Spudich JA,  Proc Natl Acad Sci U S A.  2004; 101 (45): 15825-6
• Dictyostelium myosin bipolar thick filament formation: importance of charge and specific domains of the myosin rod. Hostetter D,  Rice S, Dean S, Altman D, McMahon PM, Sutton S, Tripathy A, Spudich JA.  PLoS Biol.  2004; 2 (11): e356
• Building and using optical traps to study properties of molecular motors. Rice SE,  Purcell TJ, Spudich JA.  Methods Enzymol.  2003; 361 112-33
• Structure of an F-actin trimer disrupted by gelsolin and implications for the mechanism of severing. Dawson JF,  Sablin EP, Spudich JA, Fletterick RJ.  J Biol Chem.  2003; 278 (2): 1229-38
• Role of the lever arm in the processive stepping of myosin V. Purcell TJ,  Morris C, Spudich JA, Sweeney HL.  Proc Natl Acad Sci U S A.  2002; 99 (22): 14159-64
• Differential localization in cells of myosin II heavy chain kinases during cytokinesis and polarized migration. Liang W,  Licate L, Warrick H, Spudich J, Egelhoff T.  BMC Cell Biol.  2002; 3 19
• Quantitation of the distribution and flux of myosin-II during cytokinesis. Robinson DN,  Cavet G, Warrick HM, Spudich JA.  BMC Cell Biol.  2002; 3 4
• Dynacortin is a novel actin bundling protein that localizes to dynamic actin structures. Robinson DN,  Ocon SS, Rock RS, Spudich JA.  J Biol Chem.  2002; 277 (11): 9088-95
• The myosin swinging cross-bridge model. Spudich JA,  Nat Rev Mol Cell Biol.  2001; 2 (5): 387-92
• Myosin VI is a processive motor with a large step size. Rock RS,  Rice SE, Wells AL, Purcell TJ, Spudich JA, Sweeney HL.  Proc Natl Acad Sci U S A.  2001; 98 (24): 13655-9
• The myosin relay helix to converter interface remains intact throughout the actomyosin ATPase cycle. Shih WM,  Spudich JA.  J Biol Chem.  2001; 276 (22): 19491-4
• A myosin II mutation uncouples ATPase activity from motility and shortens step size. Murphy CT,  Rock RS, Spudich JA.  Nat Cell Biol.  2001; 3 (3): 311-5
• In vitro assays of processive myosin motors. Rock RS,  Rief M, Mehta AD, Spudich JA.  Methods.  2000; 22 (4): 373-81
• A FRET-based sensor reveals large ATP hydrolysis-induced conformational changes and three distinct states of the molecular motor myosin. Shih WM,  Gryczynski Z, Lakowicz JR, Spudich JA.  Cell.  2000; 102 (5): 683-94
• Variable surface loops and myosin activity: accessories to a motor. Murphy CT,  Spudich JA.  J Muscle Res Cell Motil.  2000; 21 (2): 139-51
• Dynacortin, a genetic link between equatorial contractility and global shape control discovered by library complementation of a Dictyostelium discoideum cytokinesis mutant. Robinson DN,  Spudich JA.  J Cell Biol.  2000; 150 (4): 823-38
• Myosin-V stepping kinetics: a molecular model for processivity. Rief M,  Rock RS, Mehta AD, Mooseker MS, Cheney RE, Spudich JA.  Proc Natl Acad Sci U S A.  2000; 97 (17): 9482-6
• Towards a molecular understanding of cytokinesis. Robinson DN,  Spudich JA.  Trends Cell Biol.  2000; 10 (6): 228-37
• Mutational analysis of phosphorylation sites in the Dictyostelium myosin II tail: disruption of myosin function by a single charge change. Nock S,  Liang W, Warrick HM, Spudich JA.  FEBS Lett.  2000; 466 (2-3): 267-72
• Biomechanics, one molecule at a time. Mehta AD,  Rief M, Spudich JA.  J Biol Chem.  1999; 274 (21): 14517-20
• The sequence of the myosin 50-20K loop affects Myosin's affinity for actin throughout the actin-myosin ATPase cycle and its maximum ATPase activity. Murphy CT,  Spudich JA.  Biochemistry.  1999; 38 (12): 3785-92
• Specialized conservation of surface loops of myosin: evidence that loops are involved in determining functional characteristics. Goodson HV,  Warrick HM, Spudich JA.  J Mol Biol.  1999; 287 (1): 173-85
• Single-molecule biomechanics with optical methods. Mehta AD,  Rief M, Spudich JA, Smith DA, Simmons RM.  Science.  1999; 283 (5408): 1689-95
• Myosin-V is a processive actin-based motor. Mehta AD,  Rock RS, Rief M, Spudich JA, Mooseker MS, Cheney RE.  Nature.  1999; 400 (6744): 590-3
• A structural model for phosphorylation control of Dictyostelium myosin II thick filament assembly. Liang W,  Warrick HM, Spudich JA.  J Cell Biol.  1999; 147 (5): 1039-48
• Use of optical traps in single-molecule study of nonprocessive biological motors. Mehta AD,  Finer JT, Spudich JA.  Methods Enzymol.  1998; 298 436-59
• Nucleotide-dependent conformational change near the fulcrum region in Dictyostelium myosin II. Liang W,  Spudich JA.  Proc Natl Acad Sci U S A.  1998; 95 (22): 12844-7
• MLCK-A, an unconventional myosin light chain kinase from Dictyostelium, is activated by a cGMP-dependent pathway. Silveira LA,  Smith JL, Tan JL, Spudich JA.  Proc Natl Acad Sci U S A.  1998; 95 (22): 13000-5
• Myosin II localization during cytokinesis occurs by a mechanism that does not require its motor domain. Zang JH,  Spudich JA.  Proc Natl Acad Sci U S A.  1998; 95 (23): 13652-7
• Single molecule biochemistry using optical tweezers. Mehta AD,  Pullen KA, Spudich JA.  FEBS Lett.  1998; 430 (1-2): 23-7
• Reflections of a lucid dreamer: optical trap design considerations. Mehta AD,  Finer JT, Spudich JA.  Methods Cell Biol.  1998; 55 47-69
• Dictyostelium myosin 25-50K loop substitutions specifically affect ADP release rates. Murphy CT,  Spudich JA.  Biochemistry.  1998; 37 (19): 6738-44
• Conditional loss-of-myosin-II-function mutants reveal a position in the tail that is critical for filament nucleation. Moores SL,  Spudich JA.  Mol Cell.  1998; 1 (7): 1043-50
• Kinetic characterization of myosin head fragments with long-lived myosin.ATP states. Friedman AL,  Geeves MA, Manstein DJ, Spudich JA.  Biochemistry.  1998; 37 (27): 9679-87
• Myosin heavy chain phosphorylation sites regulate myosin localization during cytokinesis in live cells. Sabry JH,  Moores SL, Ryan S, Zang JH, Spudich JA.  Mol Biol Cell.  1997; 8 (12): 2605-15
• On the role of myosin-II in cytokinesis: division of Dictyostelium cells under adhesive and nonadhesive conditions. Zang JH,  Cavet G, Sabry JH, Wagner P, Moores SL, Spudich JA.  Mol Biol Cell.  1997; 8 (12): 2617-29
• Detection of single-molecule interactions using correlated thermal diffusion. Mehta AD,  Finer JT, Spudich JA.  Proc Natl Acad Sci U S A.  1997; 94 (15): 7927-31
• Reversible, site-specific immobilization of polyarginine-tagged fusion proteins on mica surfaces. Nock S,  Spudich JA, Wagner P.  FEBS Lett.  1997; 414 (2): 233-8
• Phenotypically selected mutations in myosin's actin binding domain demonstrate intermolecular contacts important for motor function. Giese KC,  Spudich JA.  Biochemistry.  1997; 36 (28): 8465-73
• Bioreactive self-assembled monolayers on hydrogen-passivated Si(111) as a new class of atomically flat substrates for biological scanning probe microscopy. Wagner P,  Nock S, Spudich JA, Volkmuth WD, Chu S, Cicero RL, Wade CP, Linford MR, Chidsey CE.  J Struct Biol.  1997; 119 (2): 189-201
• Synthetic lethality screen identifies a novel yeast myosin I gene (MYO5): myosin I proteins are required for polarization of the actin cytoskeleton. Goodson HV,  Anderson BL, Warrick HM, Pon LA, Spudich JA.  J Cell Biol.  1996; 133 (6): 1277-91
• Cold-sensitive mutations of Dictyostelium myosin heavy chain highlight functional domains of the myosin motor. Patterson B,  Spudich JA.  Genetics.  1996; 143 (2): 801-10
• Structure-function studies of the myosin motor domain: importance of the 50-kDa cleft. Ruppel KM,  Spudich JA.  Mol Biol Cell.  1996; 7 (7): 1123-36
• The Dictyostelium dual-specificity kinase splA is essential for spore differentiation. Nuckolls GH,  Osherov N, Loomis WF, Spudich JA.  Development.  1996; 122 (10): 3295-305
• Myosin light chain kinase (MLCK) gene disruption in Dictyostelium: a role for MLCK-A in cytokinesis and evidence for multiple MLCKs. Smith JL,  Silveira LA, Spudich JA.  Proc Natl Acad Sci U S A.  1996; 93 (22): 12321-6
• Activation of Dictyostelium myosin light chain kinase A by phosphorylation of Thr166. Smith JL,  Silveira LA, Spudich JA.  EMBO J.  1996; 15 (22): 6075-83
• Myosin dynamics in live Dictyostelium cells. Moores SL,  Sabry JH, Spudich JA.  Proc Natl Acad Sci U S A.  1996; 93 (1): 443-6
• Structure-function analysis of the motor domain of myosin. Ruppel KM,  Spudich JA.  Annu Rev Cell Dev Biol.  1996; 12 543-73
• A novel positive selection for identifying cold-sensitive myosin II mutants in Dictyostelium. Patterson B,  Spudich JA.  Genetics.  1995; 140 (2): 505-15
• Characterization of single actin-myosin interactions. Finer JT,  Mehta AD, Spudich JA.  Biophys J.  1995; 68 (4 Suppl): 291S-296S; discussion 296S-297S
• Myosin structure/function: a combined mutagenesis-crystallography approach. Ruppel KM,  Lorenz M, Spudich JA.  Curr Opin Struct Biol.  1995; 5 (2): 181-6
• Myosin motor function: structural and mutagenic approaches. Ruppel KM,  Spudich JA.  Curr Opin Cell Biol.  1995; 7 (1): 89-93
• Myosin structure and function. Spudich JA,  Finer J, Simmons B, Ruppel K, Patterson B, Uyeda T.  Cold Spring Harb Symp Quant Biol.  1995; 60 783-91
• Identification and molecular characterization of a yeast myosin I. Goodson HV,  Spudich JA.  Cell Motil Cytoskeleton.  1995; 30 (1): 73-84
• How molecular motors work. Spudich JA,  Nature.  1994; 372 (6506): 515-8
• Molecular genetic truncation analysis of filament assembly and phosphorylation domains of Dictyostelium myosin heavy chain. Lee RJ,  Egelhoff TT, Spudich JA.  J Cell Sci.  1994; 107 ( Pt 10) 2875-86
• Stage-specific requirement for myosin II during Dictyostelium development. Springer ML,  Patterson B, Spudich JA.  Development.  1994; 120 (9): 2651-60
• Role of highly conserved lysine 130 of myosin motor domain. In vivo and in vitro characterization of site specifically mutated myosin. Ruppel KM,  Uyeda TQ, Spudich JA.  J Biol Chem.  1994; 269 (29): 18773-80
• Enzymatic activities correlate with chimaeric substitutions at the actin-binding face of myosin. Uyeda TQ,  Ruppel KM, Spudich JA.  Nature.  1994; 368 (6471): 567-9
• Single myosin molecule mechanics: piconewton forces and nanometre steps. Finer JT,  Simmons RM, Spudich JA.  Nature.  1994; 368 (6467): 113-9
• A functional recombinant myosin II lacking a regulatory light chain-binding site. Uyeda TQ,  Spudich JA.  Science.  1993; 262 (5141): 1867-70
• Dictyostelium myosin heavy chain phosphorylation sites regulate myosin filament assembly and localization in vivo. Egelhoff TT,  Lee RJ, Spudich JA.  Cell.  1993; 75 (2): 363-71
• In vitro methods for measuring force and velocity of the actin-myosin interaction using purified proteins. Warrick HM,  Simmons RM, Finer JT, Uyeda TQ, Chu S, Spudich JA.  Methods Cell Biol.  1993; 39 1-21
• Molecular evolution of the myosin family: relationships derived from comparisons of amino acid sequences. Goodson HV,  Spudich JA.  Proc Natl Acad Sci U S A.  1993; 90 (2): 659-63
• The unconventional myosin encoded by the myoA gene plays a role in Dictyostelium motility. Titus MA,  Wessels D, Spudich JA, Soll D.  Mol Biol Cell.  1993; 4 (2): 233-46
• A Dictyostelium myosin II lacking a proximal 58-kDa portion of the tail is functional in vitro and in vivo. Kubalek EW,  Uyeda TQ, Spudich JA.  Mol Biol Cell.  1992; 3 (12): 1455-62
• Control of nonmuscle myosins by phosphorylation. Tan JL,  Ravid S, Spudich JA.  Annu Rev Biochem.  1992; 61 721-59
• Yeast actin filaments display ATP-dependent sliding movement over surfaces coated with rabbit muscle myosin. Kron SJ,  Drubin DG, Botstein D, Spudich JA.  Proc Natl Acad Sci U S A.  1992; 89 (10): 4466-70
• Membrane-bound Dictyostelium myosin heavy chain kinase: a developmentally regulated substrate-specific member of the protein kinase C family. Ravid S,  Spudich JA.  Proc Natl Acad Sci U S A.  1992; 89 (13): 5877-81
• Quantized velocities at low myosin densities in an in vitro motility assay. Uyeda TQ,  Warrick HM, Kron SJ, Spudich JA.  Nature.  1991; 352 (6333): 307-11
• Molecular genetics of cell migration: Dictyostelium as a model system. Egelhoff TT,  Spudich JA.  Trends Genet.  1991; 7 (5): 161-6
• Spatial and temporal control of nonmuscle myosin localization: identification of a domain that is necessary for myosin filament disassembly in vivo. Egelhoff TT,  Brown SS, Spudich JA.  J Cell Biol.  1991; 112 (4): 677-88
• Characterization and bacterial expression of the Dictyostelium myosin light chain kinase cDNA. Identification of an autoinhibitory domain. Tan JL,  Spudich JA.  J Biol Chem.  1991; 266 (24): 16044-9
• An approach to reconstituting motility of single myosin molecules. Kron SJ,  Uyeda TQ, Warrick HM, Spudich JA.  J Cell Sci Suppl.  1991; 14 129-33
• Molecular genetic approaches to the cytoskeleton in Dictyostelium. Patterson B,  Ruppel KM, Spudich JA.  Curr Opin Genet Dev.  1991; 1 (3): 378-82
• Purification of a functional recombinant myosin fragment from Dictyostelium discoideum. Ruppel KM,  Egelhoff TT, Spudich JA.  Ann N Y Acad Sci.  1990; 582 147-55
• Genetically engineered truncated myosin in Dictyostelium: the carboxyl-terminal regulatory domain is not required for the developmental cycle. O'Halloran TJ,  Spudich JA.  Proc Natl Acad Sci U S A.  1990; 87 (20): 8110-4
• The myosin step size: measurement of the unit displacement per ATP hydrolyzed in an in vitro assay. Toyoshima YY,  Kron SJ, Spudich JA.  Proc Natl Acad Sci U S A.  1990; 87 (18): 7130-4
• Dictyostelium myosin light chain kinase. Purification and characterization. Tan JL,  Spudich JA.  J Biol Chem.  1990; 265 (23): 13818-24
• Myosin step size. Estimation from slow sliding movement of actin over low densities of heavy meromyosin. Uyeda TQ,  Kron SJ, Spudich JA.  J Mol Biol.  1990; 214 (3): 699-710
• Developmentally regulated protein-tyrosine kinase genes in Dictyostelium discoideum. Tan JL,  Spudich JA.  Mol Cell Biol.  1990; 10 (7): 3578-83
• Complementation of myosin null mutants in Dictyostelium discoideum by direct functional selection. Egelhoff TT,  Manstein DJ, Spudich JA.  Dev Biol.  1990; 137 (2): 359-67
• Molecular genetics: a key to the cytoskeleton's closet. Titus MA,  Warrick HM, Spudich JA.  Curr Opin Cell Biol.  1990; 2 (1): 116-20
• Expression of Dictyostelium myosin tail segments in Escherichia coli: domains required for assembly and phosphorylation. O'Halloran TJ,  Ravid S, Spudich JA.  J Cell Biol.  1990; 110 (1): 63-70
• Expression and characterization of a functional myosin head fragment in Dictyostelium discoideum. Manstein DJ,  Ruppel KM, Spudich JA.  Science.  1989; 246 (4930): 656-8
• Multiple actin-based motor genes in Dictyostelium. Titus MA,  Warrick HM, Spudich JA.  Cell Regul.  1989; 1 (1): 55-63
• In pursuit of myosin function. Spudich JA,  Cell Regul.  1989; 1 (1): 1-11
• Capping of surface receptors and concomitant cortical tension are generated by conventional myosin. Pasternak C,  Spudich JA, Elson EL.  Nature.  1989; 341 (6242): 549-51
• Bidirectional movement of actin filaments along tracks of myosin heads. Toyoshima YY,  Toyoshima C, Spudich JA.  Nature.  1989; 341 (6238): 154-6
• Myosin heavy chain kinase from developed Dictyostelium cells. Purification and characterization. Ravid S,  Spudich JA.  J Biol Chem.  1989; 264 (25): 15144-50
• Gene replacement in Dictyostelium: generation of myosin null mutants. Manstein DJ,  Titus MA, De Lozanne A, Spudich JA.  EMBO J.  1989; 8 (3): 923-32
• Hygromycin resistance as a selectable marker in Dictyostelium discoideum. Egelhoff TT,  Brown SS, Manstein DJ, Spudich JA.  Mol Cell Biol.  1989; 9 (5): 1965-8
• Intermolecular versus intramolecular interactions of Dictyostelium myosin: possible regulation by heavy chain phosphorylation. Pasternak C,  Flicker PF, Ravid S, Spudich JA.  J Cell Biol.  1989; 109 (1): 203-10
• Codon preference in Dictyostelium discoideum. Warrick HM,  Spudich JA.  Nucleic Acids Res.  1988; 16 (14A): 6617-35
• Expression in Escherichia coli of a functional Dictyostelium myosin tail fragment. De Lozanne A,  Berlot CH, Leinwand LA, Spudich JA.  J Cell Biol.  1987; 105 (6 Pt 2): 2999-3005
• Myosin structure and function in cell motility. Warrick HM,  Spudich JA.  Annu Rev Cell Biol.  1987; 3 379-421