Key Documents

Stanley N. Cohen, MD

Professor, Genetics
Professor, Medicine
Member, Bio-X
Member, Cancer Center

Contact Information

Clinical Offices

Clinical Pharmacology 300 Pasteur Dr M320,MC 5120 Stanford, CA 94305
Telephone Work (650) 723-5315 Fax (650) 725-1536

Postdoctoral Advisees

Ran Chen, Ning Deng, Sun Young Jeong, Johan Kers, Kwang-sun Kim, Anastasia Levitin, Mikhail Martchenko, Min Song

Web Site Links

S.N.Cohen Lab Web Site

Industry Relationships

Stanford is committed to ethical and transparent interactions with our industry partners. It is our policy to disclose payments of $5,000 or more, equity valued at $5,000 or more in a publicly traded company, or any equity in a privately held company, to physicians and scientists employed by Stanford University from companies or other commercial entities with which they interact as part of their professional activities.

Consulting: Charter Life Sciences, FivePrime Therapeutics, Functional Genetics, Intercell Therapuetics
Equity: Charter Life Sciences, Functional Genetics
Service on Board of Directors: Functional Genetics

Research Interests

Certain of our investigations are aimed at elucidating the signals that govern RNA decay. We use E. coli to investigate the mechanism of action of specific ribonucleases and related proteins, and to identify RNA substrate signals that govern stability. We are also interested in the developmental aspects of RNA stability, and study mechanisms that lead to changes in decay during morphological and biochemical differentiation in the developmentally complex bacterial genus, Streptomyces. Recently, we have begun to use a novel approach, random homozygous knockout (RHKO; see below), to identify components of RNA decay pathways in eukaryotic cells.

Our lab has long been interested in the mechanisms that lead to the evolution and dissemination of antibiotic resistance, and currently, we continue to pursue these interests by investigating the biology of linear plasmids of Streptomyces. In particular, we are interested in the mechanisms by which these plasmids interact with chromosomes to acquire and transfer genes, how plasmids evolve and undergo alterations in structure and how their telomeres function to facilitate these events and propagate plasmid DNA. Studies using DNA microarrays are aimed directly at understanding genetic pathways that regulate antibiotic production and resistance.

Some members of our lab use RHKO (random homozygous knockout) and related approaches, together with DNA microarray analysis, to investigate processes that limit the growth of mammalian cells. RHKO, a genetic method we developed to inactivate mammalian cell genes randomly and homozygously, enables the identification of genes whose loss of function leads to altered growth properties. The TSG101 gene, which was the first gene we identified using RHKO, has now been found to affect endocytic processes as well as established pathways that regulate cell division, and remains an important subject of study in our lab.

A small bioinformatics team within our research group has developed and implemented GABRIEL to aid our microarray analyses. GABRIEL (Genetic Analysis By Rules Incorporating Expert Logic) is a novel system of computer programs that incorporates expert knowledge into rules and statistical algorithms used for analysis of genetic data.

Publications

Identification of cellular genes affecting the infectivity of foot-and-mouth disease virus. Piccone ME, Feng Y, Chang AC, Mosseri R, Lu Q, Kutish GF, Lu Z, Burrage TG, Gooch C, Rock DL, Cohen SN. J Virol. 2009; 83 (13): 6681-8
Autoregulation of AbsB (RNase III) expression in Streptomyces coelicolor by endoribonucleolytic cleavage of absB operon transcripts. Xu W, Huang J, Cohen SN. J Bacteriol. 2008; 190 (15): 5526-30
YmdB: a stress-responsive ribonuclease-binding regulator of E. coli RNase III activity. Kim KS, Manasherob R, Cohen SN. Genes Dev. 2008; 22 (24): 3497-508
Genomic expression profiling of TNF-alpha-treated BDC2.5 diabetogenic CD4+ T cells. Lee LF, Lih CJ, Huang CJ, Cao T, Cohen SN, McDevitt HO. Proc Natl Acad Sci U S A. 2008; 105 (29): 10107-12
Identification of amino acid residues in the catalytic domain of RNase E essential for survival of Escherichia coli: functional analysis of DNase I subdomain. Shin E, Go H, Yeom JH, Won M, Bae J, Han SH, Han K, Lee Y, Ha NC, Moore CJ, Sohlberg B, Cohen SN, Lee K. Genetics. 2008; 179 (4): 1871-9
Inhibition of HIV budding by a genetically selected cyclic peptide targeting the Gag-TSG101 interaction. Tavassoli A, Lu Q, Gam J, Pan H, Benkovic SJ, Cohen SN. ACS Chem Biol. 2008; 3 (12): 757-64
Suppression of human tumor cell proliferation by Smurf2-induced senescence. Zhang H, Teng Y, Kong Y, Kowalski PE, Cohen SN. J Cell Physiol. 2008; 215 (3): 613-20
Glioma cells on the run - the migratory transcriptome of 10 human glioma cell lines. Demuth T, Rennert JL, Hoelzinger DB, Reavie LB, Nakada M, Beaudry C, Nakada S, Anderson EM, Henrichs AN, McDonough WS, Holz D, Joy A, Lin R, Pan KH, Lih CJ, Cohen SN, Berens ME. BMC Genomics. 2008; 9 54
Interspecies DNA microarray analysis identifies WblA as a pleiotropic down-regulator of antibiotic biosynthesis in Streptomyces. Kang SH, Huang J, Lee HN, Hur YA, Cohen SN, Kim ES. J Bacteriol. 2007; 189 (11): 4315-9
Human MDM2 isoforms translated differentially on constitutive versus p53-regulated transcripts have distinct functions in the p53/MDM2 and TSG101/MDM2 feedback control loops. Cheng TH, Cohen SN. Mol Cell Biol. 2007; 27 (1): 111-9
Characterization of a large, stable, high-copy-number Streptomyces plasmid that requires stability and transfer functions for heterologous polyketide overproduction. Fong R, Vroom JA, Hu Z, Hutchinson CR, Huang J, Cohen SN, Cohen S, Kao CM, Kao C. Appl Environ Microbiol. 2007; 73 (4): 1296-307
The LDL receptor-related protein LRP6 mediates internalization and lethality of anthrax toxin. Wei W, Lu Q, Chaudry GJ, Leppla SH, Cohen SN. Cell. 2006; 124 (6): 1141-54
RNase E maintenance of proper FtsZ/FtsA ratio required for nonfilamentous growth of Escherichia coli cells but not for colony-forming ability. Tamura M, Lee K, Miller CA, Moore CJ, Shirako Y, Kobayashi M, Cohen SN. J Bacteriol. 2006; 188 (14): 5145-52
Txr1: a transcriptional regulator of thrombospondin-1 that modulates cellular sensitivity to taxanes. Lih CJ, Wei W, Cohen SN. Genes Dev. 2006; 20 (15): 2082-95
Retention of core catalytic functions by a conserved minimal ribonuclease E peptide that lacks the domain required for tetramer formation. Caruthers JM, Feng Y, McKay DB, Cohen SN. J Biol Chem. 2006; 281 (37): 27046-51
Phenotype-based identification of host genes required for replication of African swine fever virus. Chang AC, Zsak L, Feng Y, Mosseri R, Lu Q, Kowalski P, Zsak A, Burrage TG, Neilan JG, Kutish GF, Lu Z, Laegreid W, Rock DL, Cohen SN. J Virol. 2006; 80 (17): 8705-17
rag genes: novel components of the RamR regulon that trigger morphological differentiation in Streptomyces coelicolor. San Paolo S, Huang J, Cohen SN, Thompson CJ. Mol Microbiol. 2006; 61 (5): 1167-86
Differential modulation of E. coli mRNA abundance by inhibitory proteins that alter the composition of the degradosome. Gao J, Lee K, Zhao M, Qiu J, Zhan X, Saxena A, Moore CJ, Cohen SN, Georgiou G. Mol Microbiol. 2006; 61 (2): 394-406
Effects of threshold choice on biological conclusions reached during analysis of gene expression by DNA microarrays. Pan KH, Lih CJ, Cohen SN. Proc Natl Acad Sci U S A. 2005; 102 (25): 8961-5
An integrated data analysis approach to characterize genes highly expressed in hepatocellular carcinoma. Patil MA, Chua MS, Pan KH, Lin R, Lih CJ, Cheung ST, Ho C, Li R, Fan ST, Cohen SN, Chen X, So S. Oncogene. 2005; 24 (23): 3737-47
Regional organization of gene expression in Streptomyces coelicolor. Karoonuthaisiri N, Weaver D, Huang J, Cohen SN, Kao CM. Gene. 2005; 353 (1): 53-66
Cross-regulation among disparate antibiotic biosynthetic pathways of Streptomyces coelicolor. Huang J, Shi J, Molle V, Sohlberg B, Weaver D, Bibb MJ, Karoonuthaisiri N, Lih CJ, Kao CM, Buttner MJ, Cohen SN. Mol Microbiol. 2005; 58 (5): 1276-87
Global analysis of Escherichia coli RNA degradosome function using DNA microarrays. Bernstein JA, Lin PH, Cohen SN, Lin-Chao S. Proc Natl Acad Sci U S A. 2004; 101 (9): 2758-63
Genome plasticity in Streptomyces: identification of 1 Mb TIRs in the S. coelicolor A3(2) chromosome. Weaver D, Karoonuthaisiri N, Tsai HH, Huang CH, Ho ML, Gai S, Patel KG, Huang J, Cohen SN, Hopwood DA, Chen CW, Kao CM. Mol Microbiol. 2004; 51 (6): 1535-50
Disparate effects of telomere attrition on gene expression during replicative senescence of human mammary epithelial cells cultured under different conditions. Zhang H, Herbert BS, Pan KH, Shay JW, Cohen SN. Oncogene. 2004; 23 (37): 6193-8
Reverse transcriptase activity innate to DNA polymerase I and DNA topoisomerase I proteins of Streptomyces telomere complex. Bao K, Cohen SN. Proc Natl Acad Sci U S A. 2004; 101 (40): 14361-6
SOS response induction by beta-lactams and bacterial defense against antibiotic lethality. Miller C, Thomsen LE, Gaggero C, Mosseri R, Ingmer H, Cohen SN. Science. 2004; 305 (5690): 1629-31
EST-based genome-wide gene inactivation identifies ARAP3 as a host protein affecting cellular susceptibility to anthrax toxin. Lu Q, Wei W, Kowalski PE, Chang AC, Cohen SN. Proc Natl Acad Sci U S A. 2004; 101 (49): 17246-51
Smurf2 up-regulation activates telomere-dependent senescence. Zhang H, Cohen SN. Genes Dev. 2004; 18 (24): 3028-40
Tsg101 control of human immunodeficiency virus type 1 Gag trafficking and release. Goff A, Ehrlich LS, Cohen SN, Carter CA. J Virol. 2003; 77 (17): 9173-82
Recruitment of terminal protein to the ends of Streptomyces linear plasmids and chromosomes by a novel telomere-binding protein essential for linear DNA replication. Bao K, Cohen SN. Genes Dev. 2003; 17 (6): 774-85
Senescence-specific gene expression fingerprints reveal cell-type-dependent physical clustering of up-regulated chromosomal loci. Zhang H, Pan KH, Cohen SN. Proc Natl Acad Sci U S A. 2003; 100 (6): 3251-6
The Streptomyces coelicolor polynucleotide phosphorylase homologue, and not the putative poly(A) polymerase, can polyadenylate RNA. Sohlberg B, Huang J, Cohen SN. J Bacteriol. 2003; 185 (24): 7273-8
Identification and characterization of a pSLA2 plasmid locus required for linear DNA replication and circular plasmid stable inheritance in Streptomyces lividans. Qin Z, Shen M, Cohen SN. J Bacteriol. 2003; 185 (22): 6575-82
The chaplins: a family of hydrophobic cell-surface proteins involved in aerial mycelium formation in Streptomyces coelicolor. Elliot MA, Karoonuthaisiri N, Huang J, Bibb MJ, Cohen SN, Kao CM, Buttner MJ. Genes Dev. 2003; 17 (14): 1727-40
TSG101 interaction with HRS mediates endosomal trafficking and receptor down-regulation. Lu Q, Hope LW, Brasch M, Reinhard C, Cohen SN. Proc Natl Acad Sci U S A. 2003; 100 (13): 7626-31
RraA. a protein inhibitor of RNase E activity that globally modulates RNA abundance in E. coli. Lee K, Zhan X, Gao J, Qiu J, Feng Y, Meganathan R, Cohen SN, Georgiou G. Cell. 2003; 114 (5): 623-34
DpiA binding to the replication origin of Escherichia coli plasmids and chromosomes destabilizes plasmid inheritance and induces the bacterial SOS response. Miller C, Ingmer H, Thomsen LE, Skarstad K, Cohen SN. J Bacteriol. 2003; 185 (20): 6025-31
A Streptomyces coelicolor functional orthologue of Escherichia coli RNase E shows shuffling of catalytic and PNPase-binding domains. Lee K, Cohen SN. Mol Microbiol. 2003; 48 (2): 349-60
Polyadenylation can regulate ColE1 type plasmid copy number independently of any effect on RNAI decay by decreasing the interaction of antisense RNAI with its RNAII target. Xu FF, Gaggero C, Cohen SN. Plasmid. 2002; 48 (1): 49-58
Negative regulation of cell growth and differentiation by TSG101 through association with p21(Cip1/WAF1). Oh H, Mammucari C, Nenci A, Cabodi S, Cohen SN, Dotto GP. Proc Natl Acad Sci U S A. 2002; 99 (8): 5430-5
The catalytic domain of RNase E shows inherent 3' to 5' directionality in cleavage site selection. Feng Y, Vickers TA, Cohen SN. Proc Natl Acad Sci U S A. 2002; 99 (23): 14746-51
Analysis of DNA microarrays using algorithms that employ rule-based expert knowledge. Pan KH, Lih CJ, Cohen SN. Proc Natl Acad Sci U S A. 2002; 99 (4): 2118-23
RNase G complementation of rne null mutation identifies functional interrelationships with RNase E in Escherichia coli. Lee K, Bernstein JA, Cohen SN. Mol Microbiol. 2002; 43 (6): 1445-56
Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Proc Natl Acad Sci U S A. 2002; 99 (15): 9697-702
Isolation and properties of Gas8, a growth arrest-specific gene regulated during male gametogenesis to produce a protein associated with the sperm motility apparatus. Yeh SD, Chen YJ, Chang AC, Ray R, She BR, Lee WS, Chiang HS, Cohen SN, Lin-Chao S. J Biol Chem. 2002; 277 (8): 6311-7
Survival mechanisms for Streptomyces linear replicons after telomere damage. Qin Z, Cohen SN. Mol Microbiol. 2002; 45 (3): 785-94
Global analysis of growth phase responsive gene expression and regulation of antibiotic biosynthetic pathways in Streptomyces coelicolor using DNA microarrays. Huang J, Lih CJ, Pan KH, Cohen SN. Genes Dev. 2001; 15 (23): 3183-92
RNA degradosomes exist in vivo in Escherichia coli as multicomponent complexes associated with the cytoplasmic membrane via the N-terminal region of ribonuclease E. Liou GG, Jane WN, Cohen SN, Lin NS, Lin-Chao S. Proc Natl Acad Sci U S A. 2001; 98 (1): 63-8
p53 accumulation, defective cell proliferation, and early embryonic lethality in mice lacking tsg101. Ruland J, Sirard C, Elia A, MacPherson D, Wakeham A, Li L, de la Pompa JL, Cohen SN, Mak TW. Proc Natl Acad Sci U S A. 2001; 98 (4): 1859-64
Unraveling the essential role in conjugation of the Tra protein of Streptomyces lividans plasmid pIJ101. Pettis GS, Cohen SN. Antonie Van Leeuwenhoek. 2001; 79 (3-4): 247-50
A TSG101/MDM2 regulatory loop modulates MDM2 degradation and MDM2/p53 feedback control. Li L, Liao J, Ruland J, Mak TW, Cohen SN. Proc Natl Acad Sci U S A. 2001; 98 (4): 1619-24
Terminal proteins essential for the replication of linear plasmids and chromosomes in Streptomyces. Bao K, Cohen SN. Genes Dev. 2001; 15 (12): 1518-27
Escherichia coli poly(A)-binding proteins that interact with components of degradosomes or impede RNA decay mediated by polynucleotide phosphorylase and RNase E. Feng Y, Huang H, Liao J, Cohen SN. J Biol Chem. 2001; 276 (34): 31651-6
Effects of 3' terminus modifications on mRNA functional decay during in vitro protein synthesis. Lee K, Cohen SN. J Biol Chem. 2001; 276 (26): 23268-74
Modulation of actinorhodin biosynthesis in Streptomyces lividans by glucose repression of afsR2 gene transcription. Kim ES, Hong HJ, Choi CY, Cohen SN. J Bacteriol. 2001; 183 (7): 2198-203
The RepA protein of plasmid pSC101 controls Escherichia coli cell division through the SOS response. Ingmer H, Miller C, Cohen SN. Mol Microbiol. 2001; 42 (2): 519-26
Antisense RNA-mediated deficiency of the calpain protease, nCL-4, in NIH3T3 cells is associated with neoplastic transformation and tumorigenesis. Liu K, Li L, Cohen SN. J Biol Chem. 2000; 275 (40): 31093-8
Long palindromes formed in Streptomyces by nonrecombinational intra-strand annealing. Qin Z, Cohen SN. Genes Dev. 2000; 14 (14): 1789-96
Mutational analysis of the tra locus of the broad-host-range Streptomyces plasmid pIJ101. Pettis GS, Cohen SN. J Bacteriol. 2000; 182 (16): 4500-4
Neoplastic transformation and tumorigenesis associated with sam68 protein deficiency in cultured murine fibroblasts. Liu K, Li L, Nisson PE, Gruber C, Jessee J, Cohen SN. J Biol Chem. 2000; 275 (51): 40195-201
Unpaired terminal nucleotides and 5' monophosphorylation govern 3' polyadenylation by Escherichia coli poly(A) polymerase I. Feng Y, Cohen SN. Proc Natl Acad Sci U S A. 2000; 97 (12): 6415-20
TSG101 protein steady-state level is regulated posttranslationally by an evolutionarily conserved COOH-terminal sequence. Feng GH, Lih CJ, Cohen SN. Cancer Res. 2000; 60 (6): 1736-41
Alternative splicing regulates the production of ARD-1 endoribonuclease and NIPP-1, an inhibitor of protein phosphatase-1, as isoforms encoded by the same gene. Chang AC, Sohlberg B, Trinkle-Mulcahy L, Claverie-Martin F, Cohen P, Cohen SN. Gene. 1999; 240 (1): 45-55
Reversible tumorigenesis induced by deficiency of vasodilator-stimulated phosphoprotein. Liu K, Li L, Nisson PE, Gruber C, Jessee J, Cohen SN. Mol Cell Biol. 1999; 19 (5): 3696-703
Nuclear organisation of NIPP1, a regulatory subunit of protein phosphatase 1 that associates with pre-mRNA splicing factors. Trinkle-Mulcahy L, Ajuh P, Prescott A, Claverie-Martin F, Cohen S, Lamond AI, Cohen P. J Cell Sci. 1999; 112 ( Pt 2) 157-68
Tumor susceptibility gene 101 protein represses androgen receptor transactivation and interacts with p300. Sun Z, Pan J, Hope WX, Cohen SN, Balk SP. Cancer. 1999; 86 (4): 689-96
Regulation of transfer functions by the imp locus of the Streptomyces coelicolor plasmidogenic element SLP1. Hagege JM, Brasch MA, Cohen SN. J Bacteriol. 1999; 181 (19): 5976-83
Separate roles of Escherichia coli replication proteins in synthesis and partitioning of pSC101 plasmid DNA. Miller C, Cohen SN. J Bacteriol. 1999; 181 (24): 7552-7
Poly(A)- and poly(U)-specific RNA 3' tail shortening by E. coli ribonuclease E. Huang H, Liao J, Cohen SN. Nature. 1998; 391 (6662): 99-102
Destabilized inheritance of pSC101 and other Escherichia coli plasmids by DpiA, a novel two-component system regulator. Ingmer H, Miller CA, Cohen SN. Mol Microbiol. 1998; 29 (1): 49-59
Replication at the telomeres of the Streptomyces linear plasmid pSLA2. Qin Z, Cohen SN. Mol Microbiol. 1998; 28 (5): 893-903
Cell cycle-dependent subcellular localization of the TSG101 protein and mitotic and nuclear abnormalities associated with TSG101 deficiency. Xie W, Li L, Cohen SN. Proc Natl Acad Sci U S A. 1998; 95 (4): 1595-600
gas7: A gene expressed preferentially in growth-arrested fibroblasts and terminally differentiated Purkinje neurons affects neurite formation. Ju YT, Chang AC, She BR, Tsaur ML, Hwang HM, Chao CC, Cohen SN, Lin-Chao S. Proc Natl Acad Sci U S A. 1998; 95 (19): 11423-8
Aberrant splicing of the TSG101 and FHIT genes occurs frequently in multiple malignancies and in normal tissues and mimics alterations previously described in tumours. Gayther SA, Barski P, Batley SJ, Li L, de Foy KA, Cohen SN, Ponder BA, Caldas C. Oncogene. 1997; 15 (17): 2119-26
The TSG101 tumor susceptibility gene is located in chromosome 11 band p15 and is mutated in human breast cancer. Li L, Li X, Francke U, Cohen SN. Cell. 1997; 88 (1): 143-54
ARD-1 cDNA from human cells encodes a site-specific single-strand endoribonuclease that functionally resembles Escherichia coli RNase E. Claverie-Martin F, Wang M, Cohen SN. J Biol Chem. 1997; 272 (21): 13823-8
A developmentally regulated Streptomyces endoribonuclease resembles ribonuclease E of Escherichia coli. Hagège JM, Cohen SN. Mol Microbiol. 1997; 25 (6): 1077-90
RNase E: still a wonderfully mysterious enzyme. Cohen SN, McDowall KJ. Mol Microbiol. 1997; 23 (6): 1099-106
The N-terminal domain of the rne gene product has RNase E activity and is non-overlapping with the arginine-rich RNA-binding site. McDowall KJ, Cohen SN. J Mol Biol. 1996; 255 (3): 349-55
Plasmid transfer and expression of the transfer (tra) gene product of plasmid pIJ101 are temporally regulated during the Streptomyces lividans life cycle. Pettis GS, Cohen SN. Mol Microbiol. 1996; 19 (5): 1127-35
Tsg101: a novel tumor susceptibility gene isolated by controlled homozygous functional knockout of allelic loci in mammalian cells. Li L, Cohen SN. Cell. 1996; 85 (3): 319-29
Streptomyces linear plasmids that contain a phage-like, centrally located, replication origin. Chang PC, Kim ES, Cohen SN. Mol Microbiol. 1996; 22 (5): 789-800
The platelet-derived growth factor alpha-receptor is encoded by a growth-arrest-specific (gas) gene. Lih CJ, Cohen SN, Wang C, Lin-Chao S. Proc Natl Acad Sci U S A. 1996; 93 (10): 4617-22
Monomer-dimer equilibrium of the pSC101 RepA protein. Ingmer H, Fong EL, Cohen SN. J Mol Biol. 1995; 250 (3): 309-14
Boundaries of the pSC101 minimal replicon are conditional. Miller CA, Ingmer H, Cohen SN. J Bacteriol. 1995; 177 (17): 4865-71
Transformed mouse cell lines that consist predominantly of cells maintaining bovine papilloma virus at high copy number. Ravnan JB, Cohen SN. Virology. 1995; 213 (2): 526-34
Disparate replication properties of integrated and extrachromosomal forms of bovine papilloma virus in ID13 cells. Ten Hagen KG, Ravnan JB, Cohen SN. J Mol Biol. 1995; 254 (2): 119-29
Isolation and characterization of plasmid mutations that enable partitioning of pSC101 replicons lacking the partition (par) locus. Conley DL, Cohen SN. J Bacteriol. 1995; 177 (4): 1086-9
Surprises at the 3' end of prokaryotic RNA. Cohen SN, Cell. 1995; 80 (6): 829-32
Site-specific RNase E cleavage of oligonucleotides and inhibition by stem-loops. McDowall KJ, Kaberdin VR, Wu SW, Cohen SN, Lin-Chao S. Nature. 1995; 374 (6519): 287-90
RNA degradation in Escherichia coli regulated by 3' adenylation and 5' phosphorylation. Xu F, Cohen SN. Nature. 1995; 374 (6518): 180-3
Effects of the pSC101 partition (par) locus on in vivo DNA supercoiling near the plasmid replication origin. Conley DL, Cohen SN. Nucleic Acids Res. 1995; 23 (4): 701-7
Sequences essential for replication of plasmid pIJ101 in Streptomyces lividans. Brasch MA, Cohen SN. Plasmid. 1995; 33 (3): 191-7
Mutations that affect regulation of the korB gene of Streptomyces lividans plasmid plJ101 alter plasmid transmission. Tai JT, Cohen SN. Mol Microbiol. 1994; 12 (1): 31-9
A+U content rather than a particular nucleotide order determines the specificity of RNase E cleavage. McDowall KJ, Lin-Chao S, Cohen SN. J Biol Chem. 1994; 269 (14): 10790-6
Bidirectional replication from an internal origin in a linear streptomyces plasmid. Chang PC, Cohen SN. Science. 1994; 265 (5174): 952-4
Transfer of the plJ101 plasmid in Streptomyces lividans requires a cis-acting function dispensable for chromosomal gene transfer. Pettis GS, Cohen SN. Mol Microbiol. 1994; 13 (6): 955-64
ard-1: a human gene that reverses the effects of temperature-sensitive and deletion mutations in the Escherichia coli rne gene and encodes an activity producing RNase E-like cleavages. Wang M, Cohen SN. Proc Natl Acad Sci U S A. 1994; 91 (22): 10591-5
afsR2: a previously undetected gene encoding a 63-amino-acid protein that stimulates antibiotic production in Streptomyces lividans. Vögtli M, Chang PC, Cohen SN. Mol Microbiol. 1994; 14 (4): 643-53
Effects of nucleotide sequence on the specificity of rne-dependent and RNase E-mediated cleavages of RNA I encoded by the pBR322 plasmid. Lin-Chao S, Wong TT, McDowall KJ, Cohen SN. J Biol Chem. 1994; 269 (14): 10797-803
The partition (par) locus of pSC101 is an enhancer of plasmid incompatibility. Miller CA, Cohen SN. Mol Microbiol. 1993; 9 (4): 695-702
The Escherichia coli pcnB gene promotes adenylylation of antisense RNAI of ColE1-type plasmids in vivo and degradation of RNAI decay intermediates. Xu F, Lin-Chao S, Cohen SN. Proc Natl Acad Sci U S A. 1993; 90 (14): 6756-60
The ams-1 and rne-3071 temperature-sensitive mutations in the ams gene are in close proximity to each other and cause substitutions within a domain that resembles a product of the Escherichia coli mre locus. McDowall KJ, Hernandez RG, Lin-Chao S, Cohen SN. J Bacteriol. 1993; 175 (13): 4245-9
Timing of replication of beta satellite repeats of human chromosomes. Ten Hagen KG, Cohen SN. Nucleic Acids Res. 1993; 21 (9): 2139-42
Excisive recombination of the SLP1 element in Streptomyces lividans is mediated by Int and enhanced by Xis. Brasch MA, Cohen SN. J Bacteriol. 1993; 175 (10): 3075-82
Activation of transcriptional fusions in Streptomyces lividans resulting from insertion of a 14-bp oligonucleotide. Brasch MA, Shiffman D, Cohen SN. Nucleic Acids Res. 1993; 21 (17): 4151
The pSC101 par locus alters protein-DNA interactions in vivo at the plasmid replication origin. Ingmer H, Cohen SN. J Bacteriol. 1993; 175 (18): 6046-8
Role of the imp operon of the Streptomyces coelicolor genetic element SLP1: two imp-encoded proteins interact to autoregulate imp expression and control plasmid maintenance. Shiffman D, Cohen SN. J Bacteriol. 1993; 175 (21): 6767-74
The active form of the KorB protein encoded by the Streptomyces plasmid pIJ101 is a processed product that binds differentially to the two promoters it regulates. Tai JT, Cohen SN. J Bacteriol. 1993; 175 (21): 6996-7005
Excess intracellular concentration of the pSC101 RepA protein interferes with both plasmid DNA replication and partitioning. Ingmer H, Cohen SN. J Bacteriol. 1993; 175 (24): 7834-41
Bacterial plasmids: their extraordinary contribution to molecular genetics. Cohen SN, Gene. 1993; 135 (1-2): 67-76
Localization and nucleotide sequences of genes mediating site-specific recombination of the SLP1 element in Streptomyces lividans. Brasch MA, Pettis GS, Lee SC, Cohen SN. J Bacteriol. 1993; 175 (10): 3067-74
Random-choice replication of extrachromosomal bovine papillomavirus (BPV) molecules in heterogeneous, clonally derived BPV-infected cell lines. Ravnan JB, Gilbert DM, Ten Hagen KG, Cohen SN. J Virol. 1992; 66 (12): 6946-52
The chromosomal integration site for the Streptomyces plasmid SLP1 is a functional tRNA(Tyr) gene essential for cell viability. Vögtli M, Cohen SN. Mol Microbiol. 1992; 6 (20): 3041-50
Reconstruction of a Streptomyces linear replicon from separately cloned DNA fragments: existence of a cryptic origin of circular replication within the linear plasmid. Shiffman D, Cohen SN. Proc Natl Acad Sci U S A. 1992; 89 (13): 6129-33
Mouse genomic DNA sequences homologous to sea urchin TU elements are genetically stable polydispersed repeats useful for analysis of multiple RFLPs. Gilbert DM, Hernandez R, Cohen SN. Genomics. 1992; 12 (2): 357-62
Propagation of pSC101 plasmids defective in binding of integration host factor. Biek DP, Cohen SN. J Bacteriol. 1992; 174 (3): 785-92
Effects of translation on degradation of mRNA segments transcribed from the polycistronic puf operon of Rhodobacter capsulatus. Klug G, Cohen SN. J Bacteriol. 1991; 173 (4): 1478-84
Gyrase-dependent stabilization of pSC101 plasmid inheritance by transcriptionally active promoters. Beaucage SL, Miller CA, Cohen SN. EMBO J. 1991; 10 (9): 2583-8
The rate of processing and degradation of antisense RNAI regulates the replication of ColE1-type plasmids in vivo. Lin-Chao S, Cohen SN. Cell. 1991; 65 (7): 1233-42
Combined actions of multiple hairpin loop structures and sites of rate-limiting endonucleolytic cleavage determine differential degradation rates of individual segments within polycistronic puf operon mRNA. Klug G, Cohen SN. J Bacteriol. 1990; 172 (9): 5140-6
Isolation and characterization of Rhodobacter capsulatus mutants defective in oxygen regulation of the puf operon. Narro ML, Adams CW, Cohen SN. J Bacteriol. 1990; 172 (8): 4549-54
Position effects on the timing of replication of chromosomally integrated simian virus 40 molecules in Chinese hamster cells. Gilbert DM, Cohen SN. Mol Cell Biol. 1990; 10 (8): 4345-55
Role of DNA superhelicity in partitioning of the pSC101 plasmid. Miller CA, Beaucage SL, Cohen SN. Cell. 1990; 62 (1): 127-33
Mutational and functional analysis of the korA and korB gene products of Streptomyces plasmid pIJ101. Stein DS, Cohen SN. Mol Gen Genet. 1990; 222 (2-3): 337-44
Replication timing of DNA sequences associated with human centromeres and telomeres. Ten Hagen KG, Gilbert DM, Willard HF, Cohen SN. Mol Cell Biol. 1990; 10 (12): 6348-55
Effects on the formation of antenna complex B870 of Rhodobacter capsulatus by exchange of charged amino acids in the N-terminal domain of the alpha and beta pigment-binding proteins. Dörge B, Klug G, Gad'on N, Cohen SN, Drews G. Biochemistry. 1990; 29 (33): 7754-8
Rapid two-stage polymerase chain reaction amplification of chromosomal DNA segments in lysates made from monolayer cultures attached to microcarrier beads. Sue LC, Brenner DG, Cohen SN. Gene. 1990; 93 (2): 293-6
Initiation of pro-opiomelanocortin mRNA from a normally quiescent promoter in a human small cell lung cancer cell line. Chang AC, Israel A, Gazdar A, Cohen SN. Gene. 1989; 84 (1): 115-26
Identification and analysis of transcriptional regulatory signals for the kil and kor loci of Streptomyces plasmid pIJ101. Stein DS, Kendall KJ, Cohen SN. J Bacteriol. 1989; 171 (11): 5768-75
Analysis of mammalian cell genetic regulation in situ by using retrovirus-derived "portable exons" carrying the Escherichia coli lacZ gene. Brenner DG, Lin-Chao S, Cohen SN. Proc Natl Acad Sci U S A. 1989; 86 (14): 5517-21
Identification and characterization of a locus inhibiting extrachromosomal maintenance of the Streptomyces plasmid SLP1. Grant SR, Lee SC, Kendall K, Cohen SN. Mol Gen Genet. 1989; 217 (2-3): 324-31
A cloned regulatory gene of Streptomyces lividans can suppress the pigment deficiency phenotype of different developmental mutants. Stein D, Cohen SN. J Bacteriol. 1989; 171 (4): 2258-61
Involvement of integration host factor (IHF) in maintenance of plasmid pSC101 in Escherichia coli: mutations in the topA gene allow pSC101 replication in the absence of IHF. Biek DP, Cohen SN. J Bacteriol. 1989; 171 (4): 2066-74
Involvement of integration host factor (IHF) in maintenance of plasmid pSC101 in Escherichia coli: characterization of pSC101 mutants that replicate in the absence of IHF. Biek DP, Cohen SN. J Bacteriol. 1989; 171 (4): 2056-65
Structural and functional analysis of transcriptional control of the Rhodobacter capsulatus puf operon. Adams CW, Forrest ME, Cohen SN, Beatty JT. J Bacteriol. 1989; 171 (1): 473-82
Site-specific insertion of biologically functional adventitious genes into the Streptomyces lividans chromosome. Omer CA, Stein D, Cohen SN. J Bacteriol. 1988; 170 (5): 2174-84
Stable inheritance of bacterial plasmids: practical considerations in the release of organisms into the environment. Cohen SN, Miller CA, Beaucage S, Biek DP. Basic Life Sci. 1988; 45 97-104
Analysis of recombination occurring at SLP1 att sites. Lee SC, Omer CA, Brasch MA, Cohen SN. J Bacteriol. 1988; 170 (12): 5806-13
An intercistronic stem-loop structure functions as an mRNA decay terminator necessary but insufficient for puf mRNA stability. Chen CY, Beatty JT, Cohen SN, Belasco JG. Cell. 1988; 52 (4): 609-19
Pleiotropic effects of localized Rhodobacter capsulatus puf operon deletions on production of light-absorbing pigment-protein complexes. Klug G, Cohen SN. J Bacteriol. 1988; 170 (12): 5814-21
Complete nucleotide sequence of the Streptomyces lividans plasmid pIJ101 and correlation of the sequence with genetic properties. Kendall KJ, Cohen SN. J Bacteriol. 1988; 170 (10): 4634-51
Plasmid transfer in Streptomyces lividans: identification of a kil-kor system associated with the transfer region of pIJ101. Kendall KJ, Cohen SN. J Bacteriol. 1987; 169 (9): 4177-83
Bovine papilloma virus plasmids replicate randomly in mouse fibroblasts throughout S phase of the cell cycle. Gilbert DM, Cohen SN. Cell. 1987; 50 (1): 59-68
Effect of premature termination of translation on mRNA stability depends on the site of ribosome release. Nilsson G, Belasco JG, Cohen SN, von Gabain A. Proc Natl Acad Sci U S A. 1987; 84 (14): 4890-4
Biological consequences of segmental alterations in mRNA stability: effects of deletion of the intercistronic hairpin loop region of the Rhodobacter capsulatus puf operon. Klug G, Adams CW, Belasco J, Doerge B, Cohen SN. EMBO J. 1987; 6 (11): 3515-20
The stability of E. coli gene transcripts is dependent on determinants localized to specific mRNA segments. Belasco JG, Nilsson G, von Gabain A, Cohen SN. Cell. 1986; 46 (2): 245-51
Identification and characterization of recD, a gene affecting plasmid maintenance and recombination in Escherichia coli. Biek DP, Cohen SN. J Bacteriol. 1986; 167 (2): 594-603
Human homologs of TU transposon sequences: polypurine/polypyrimidine sequence elements that can alter DNA conformation in vitro and in vivo. Hoffman-Liebermann B, Liebermann D, Troutt A, Kedes LH, Cohen SN. Mol Cell Biol. 1986; 6 (11): 3632-42
Structural analysis of plasmid and chromosomal loci involved in site-specific excision and integration of the SLP1 element of Streptomyces coelicolor. Omer CA, Cohen SN. J Bacteriol. 1986; 166 (3): 999-1006
Sequences from sea urchin TU transposons are conserved among multiple eucaryotic species, including humans. Liebermann D, Hoffman-Liebermann B, Troutt AB, Kedes L, Cohen SN. Mol Cell Biol. 1986; 6 (1): 218-26
Sequence of the cDNA encoding porcine pro-opiomelanocortin. Gossard FJ, Chang AC, Cohen SN. Biochim Biophys Acta. 1986; 866 (1): 68-74
Streptomyces contain Escherichia coli-type A + T-rich promoters having novel structural features. Jaurin B, Cohen SN. Gene. 1985; 39 (2-3): 191-201
Hormonally mediated negative regulation of human pro-opiomelanocortin gene expression after transfection into mouse L cells. Israel A, Cohen SN. Mol Cell Biol. 1985; 5 (9): 2443-53
Nucleotide sequences encoding and promoting expression of three antibiotic resistance genes indigenous to Streptomyces. Bibb MJ, Bibb MJ, Ward JM, Cohen SN. Mol Gen Genet. 1985; 199 (1): 26-36
TU elements: a heterogeneous family of modularly structured eucaryotic transposons. Hoffman-Liebermann B, Liebermann D, Kedes LH, Cohen SN. Mol Cell Biol. 1985; 5 (5): 991-1001
Partitioning of the pSC101 plasmid during cell division. Cohen SN, Miller CA, Tucker WT, Meacock PA, Gustafsson P. Basic Life Sci. 1985; 30 383-95
Differential expression of photosynthesis genes in R. capsulata results from segmental differences in stability within the polycistronic rxcA transcript. Belasco JG, Beatty JT, Adams CW, von Gabain A, Cohen SN. Cell. 1985; 40 (1): 171-81
Growth-rate dependent regulation of mRNA stability in Escherichia coli. Nilsson G, Belasco JG, Cohen SN, von Gabain A. Nature. 1984 Nov 1-7; 312 (5989): 75-7
Plasmid formation in Streptomyces: excision and integration of the SLP1 replicon at a specific chromosomal site. Omer CA, Cohen SN. Mol Gen Genet. 1984; 196 (3): 429-38
Structural and functional analysis of the par region of the pSC 10 1 plasmid. Tucker WT, Miller CA, Cohen SN. Cell. 1984; 38 (1): 191-201
Streptomyces lividans RNA polymerase recognizes and uses Escherichia coli transcriptional signals. Jaurin B, Cohen SN. Gene. 1984; 28 (1): 83-91
Effects of alterations in the translation control region on bacterial gene expression: use of cat gene constructs transcribed from the lac promoter as a model system. Schottel JL, Sninsky JJ, Cohen SN. Gene. 1984; 28 (2): 177-93
An unusual transposon with long terminal inverted repeats in the sea urchin Strongylocentrotus purpuratus. Liebermann D, Hoffman-Liebermann B, Weinthal J, Childs G, Maxson R, Mauron A, Cohen SN, Kedes L. Nature. 1983 Nov 24-30; 306 (5941): 342-7
Decay of mRNA in Escherichia coli: investigation of the fate of specific segments of transcripts. von Gabain A, Belasco JG, Schottel JL, Chang AC, Cohen SN. Proc Natl Acad Sci U S A. 1983; 80 (3): 653-7
Use of synthetic oligonucleotide probes complementary to genes for human HLA-DR alpha and beta as extension primers for the isolation of 5'-specific genomic clones. Das HK, Biro PA, Cohen SN, Erlich HA, von Gabain A, Lawrance SK, Lemaux PG, McDevitt HO, Peterlin BM, Schulz MF, Sood AK, Weissman SM. Proc Natl Acad Sci U S A. 1983; 80 (6): 1531-5
Alignment of genetic and restriction maps of the photosynthesis region of the Rhodopseudomonas capsulata chromosome by a conjugation-mediated marker rescue technique. Taylor DP, Cohen SN, Clark WG, Marrs BL. J Bacteriol. 1983; 154 (2): 580-90
Hybridization of cloned Rhodopseudomonas capsulata photosynthesis genes with DNA from other photosynthetic bacteria. Beatty JT, Cohen SN. J Bacteriol. 1983; 154 (3): 1440-5
Nucleotide sequence of the partition locus of Escherichia coli plasmid pSC101. Miller CA, Tucker WT, Meacock PA, Gustafsson P, Cohen SN. Gene. 1983; 24 (2-3): 309-15
Hollow-fiber membrane bioreactors using immobilized E. coli for protein synthesis. Inloes DS, Smith WJ, Taylor DP, Cohen SN, Michaels AS, Robertson CR. Biotechnol Bioeng. 1983; 25 (11): 2653-81
A DNA polymorphism in close physical linkage with the proopiomelanocortin gene. Feder J, Migone N, Chang AC, Cochet M, Cohen SN, Cann H, Cavalli-Sforza LL. Am J Hum Genet. 1983; 35 (6): 1090-6
Characterization of the structural gene and putative 5'-regulatory sequences for human proopiomelanocortin. Cochet M, Chang AC, Cohen SN. Nature. 1982; 297 (5864): 335-9
Overproduction of the Tn3 transposition protein and its role in DNA transposition. Casadaban MJ, Chou J, Cohen SN. Cell. 1982; 28 (2): 345-54
Amino-terminal sequence of the Tn3 transposase protein. Ditto MD, Chou J, Hunkapiller MW, Fennewald MA, Gerrard SP, Hood LE, Cohen SN, Casadaban MJ. J Bacteriol. 1982; 149 (1): 407-10
Gene expression in Streptomyces: construction and application of promoter-probe plasmid vectors in Streptomyces lividans. Bibb MJ, Cohen SN. Mol Gen Genet. 1982; 187 (2): 265-77
Excision of chromosomal DNA sequences from Streptomyces coelicolor forms a novel family of plasmids detectable in Streptomyces lividans. Bibb MJ, Ward JM, Kieser T, Cohen SN, Hopwood DA. Mol Gen Genet. 1981; 184 (2): 230-40
Cloning and analysis of strong promoters is made possible by the downstream placement of a RNA termination signal. Gentz R, Langner A, Chang AC, Cohen SN, Bujard H. Proc Natl Acad Sci U S A. 1981; 78 (8): 4936-40
Cloning and expression in streptomyces lividans of antibiotic resistance genes derived from Escherichia coli. Schottel JL, Bibb MJ, Cohen SN. J Bacteriol. 1981; 146 (1): 360-8
Tn3: transposition and control. Casadaban MJ, Chou J, Lemaux P, Tu CP, Cohen SN. Cold Spring Harb Symp Quant Biol. 1981; 45 Pt 1 269-73
Construction and characterization of a novel two-plasmid system for accomplishing temperature-regulated, amplified expression of cloned adventitious genes in Escherichia coli. Sninsky JJ, Uhlin BE, Gustafsson P, Cohen SN. Gene. 1981; 16 (1-3): 275-86
Initiation of protein synthesis in bacteria at a translational start codon of mamalian cDNA: effects of the preceding nucleotide sequence. Chang AC, Erlich HA, Gunsalus RP, Nunberg JH, Kaufman RJ, Schimke RT, Cohen SN. Proc Natl Acad Sci U S A. 1980; 77 (3): 1442-6
Transposable genetic elements. Cohen SN, Shapiro JA. Sci Am. 1980; 242 (2): 40-9
Translocation specificity of the Tn3 element: characterization of sites of multiple insertions. Tu CP, Cohen SN. Cell. 1980; 19 (1): 151-60
Analysis of gene control signals by DNA fusion and cloning in Escherichia coli. Casadaban MJ, Cohen SN. J Mol Biol. 1980; 138 (2): 179-207
A DNA cloning system for interspecies gene transfer in antibiotic-producing Streptomyces. Bibb M, Schottel JL, Cohen SN. Nature. 1980; 284 (5756): 526-31
Effect of DNA sequences adjacent to the termini of Tn3 on sequential translocation. Tu CP, Cohen SN. Mol Gen Genet. 1980; 177 (4): 597-601
Structure and genomic organization of the mouse dihydrofolate reductase gene. Nunberg JH, Kaufman RJ, Chang AC, Cohen SN, Schimke RT. Cell. 1980; 19 (2): 355-64
Partitioning of bacterial plasmids during cell division: a cis-acting locus that accomplishes stable plasmid inheritance. Meacock PA, Cohen SN. Cell. 1980; 20 (2): 529-42
3'-end labeling of DNA with [alpha-32P]cordycepin-5'-triphosphate. Tu CP, Cohen SN. Gene. 1980; 10 (2): 177-83
Studies of cloned DNA encoding the structure for the bovine corticotropin-beta-lipotropin precursor protein. Cohen SN, Chang AC, Nakanishi S, Inoue A, Kita T, Nakamura M, Numa S. Ann N Y Acad Sci. 1980; 343 415-24
In vitro gene fusions that join an enzymatically active beta-galactosidase segment to amino-terminal fragments of exogenous proteins: Escherichia coli plasmid vectors for the detection and cloning of translational initiation signals. Casadaban MJ, Chou J, Cohen SN. J Bacteriol. 1980; 143 (2): 971-80
Structural organization of human genomic DNA encoding the pro-opiomelanocortin peptide. Chang AC, Cochet M, Cohen SN. Proc Natl Acad Sci U S A. 1980; 77 (8): 4890-4
The transplantation and manipulation of genes in microorganisms. Cohen SN, Harvey Lect. 1980; 74 173-204
F plasmid provides a function that promotes recA-independent site-specific fusions of pSC101 replicon. Miller CA, Cohen SN. Nature. 1980; 285 (5766): 577-9
Transposition protein of Tn3: identification and characterisation of an essential repressor-controlled gene product. Chou J, Lemaux PG, Casadaban MJ, Cohen SN. Nature. 1979 Dec 20-27; 282 (5741): 801-6
Structural and functional analysis of cloned DNA segments containing the replication and incompatibility regions of a miniplasmid derived from a copy number mutant of NR1. Taylor DP, Cohen SN. J Bacteriol. 1979; 137 (1): 92-104
Lactose genes fused to exogenous promoters in one step using a Mu-lac bacteriophage: in vivo probe for transcriptional control sequences. Casadaban MJ, Cohen SN. Proc Natl Acad Sci U S A. 1979; 76 (9): 4530-3
Antimicrobial selection by a computer. A blinded evaluation by infectious diseases experts. Yu VL, Fagan LM, Wraith SM, Clancey WJ, Scott AC, Hannigan J, Blum RL, Buchanan BG, Cohen SN. JAMA. 1979; 242 (12): 1279-82
Evaluating the performance of a computer-based consultant. Yu VL, Buchanan BG, Shortliffe EH, Wraith SM, Davis R, Scott AC, Cohen SN. Comput Programs Biomed. 1979; 9 (1): 95-102
High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA. Chang S, Cohen SN. Mol Gen Genet. 1979; 168 (1): 111-5
Filter affinity transfer. A new technique for the in situ identification of proteins in gels. Erlich HA, Levinson JR, Cohen SN, McDevitt HO. J Biol Chem. 1979; 254 (23): 12240-7
Immunological detection and characterization of products translated from cloned DNA fragments. Erlich HA, Cohen SN, McDevitt HO. Methods Enzymol. 1979; 68 443-53
Genetic analysis of the inter-relationship between plasmid replication and incompatibility. Meacock PA, Cohen SN. Mol Gen Genet. 1979; 174 (2): 135-47
Cloning and endonuclease mapping of the hepatitis B viral genome. Sninsky JJ, Siddiqui A, Robinson WS, Cohen SN. Nature. 1979; 279 (5711): 346-8
Effect of temperature on translocation frequency of the Tn3 element. Kretschmer PJ, Cohen SN. J Bacteriol. 1979; 139 (2): 515-9
Computer-based system for adverse drug reaction detection and prevention. Tatro DS, Moore TN, Cohen SN. Am J Hosp Pharm. 1979; 36 (2): 198-201
Nucleotide sequence of cloned cDNA for bovine corticotropin-beta-lipotropin precursor. Nakanishi S, Inoue A, Kita T, Nakamura M, Chang AC, Cohen SN, Numa S. Nature. 1979; 278 (5703): 423-7
Studies of the specificity and control of transposition of the Tn3 element. Cohen SN, Casadaban MJ, Chou J, Tu CP. Cold Spring Harb Symp Quant Biol. 1979; 43 Pt 2 1247-55
Identification and characterization of a self-regulated repressor of translocation of the Tn3 element. Chou J, Casadaban MJ, Lemaux PG, Cohen SN. Proc Natl Acad Sci U S A. 1979; 76 (8): 4020-4
Construction of bacterial plasmids that contain the nucleotide sequence for bovine corticotropin-beta-lipotropin precursor. Nakanishi S, Inoue A, Kita T, Numa S, Chang AC, Cohen SN, Nunberg J, Schimke RT. Proc Natl Acad Sci U S A. 1978; 75 (12): 6021-5
Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase. Chang AC, Nunberg JH, Kaufman RJ, Erlich HA, Schimke RT, Cohen SN. Nature. 1978; 275 (5681): 617-24
Phenotypically cryptic EcoRI endonuclease activity specified by the ColE1 plasmid. Miller CA, Cohen SN. Proc Natl Acad Sci U S A. 1978; 75 (3): 1265-9
Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. Chang AC, Cohen SN. J Bacteriol. 1978; 134 (3): 1141-56
Cloning and characterization of EcoRI and HindIII restriction endonuclease-generated fragments of antibiotic resistance plasmids R6-5 and R6. Timmis KN, Cabello F, Cohen SN. Mol Gen Genet. 1978; 162 (2): 121-37
A sensitive radioimmunoassay for detecting products translated from cloned DNA fragments. Erlich HA, Cohen SN, McDevitt HO. Cell. 1978; 13 (4): 681-9
Instability of plasmid DNA sequences: macro and micro evolution of the antibiotic resistance plasmid R6-5. Timmis KN, Cabello F, Andrés I, Nordheim A, Burkhardt HJ, Cohen SN. Mol Gen Genet. 1978; 167 (1): 11-19
Site-specific DNA deletions occurring adjacent to the termini of a transposable ampicillin resistance element (Tn3). Nisen PD, Kopecko DJ, Chou J, Cohen SN. J Mol Biol. 1977; 117 (4): 975-8
Revised interpretation of the origin of the pSC101 plasmid. Cohen SN, Chang AC. J Bacteriol. 1977; 132 (2): 734-7
Selected translocation of plasmid genes: frequency and regional specificity of translocation of the Tn3 element. Kretschmer PJ, Cohen SN. J Bacteriol. 1977; 130 (2): 888-99
Recombinant DNA: fact and fiction. Cohen SN, Science. 1977; 195 (4279): 654-7
Recombinant DNA: fact and fiction. Cohen SN, West J Med. 1977; 126 (5): 415-20
Genetic recombination through protoplast fusion in Streptomyces. Hopwood DA, Wright HM, Bibb MJ, Cohen SN. Nature. 1977; 268 (5616): 171-4
In vivo site-specific genetic recombination promoted by the EcoRI restriction endonuclease. Chang S, Cohen SN. Proc Natl Acad Sci U S A. 1977; 74 (11): 4811-5
Receptivity of physicians in a teaching hospital to computerized drug interaction monitoring and reporting system. Morrell J, Podlone M, Cohen SN. Med Care. 1977; 15 (1): 68-78
Computerized consultation system for selection of antimicrobial therapy. Wraith SM, Aikins JS, Buchanan BG, Clancey WJ, Davis R, Fagan LM, Hannigan JF, Scott AC, Shortliffe EH, van Melle WJ, Yu VL, Axline SG, Cohen SN. Am J Hosp Pharm. 1976; 33 (12): 1304-8
Covalently closed circular DNA molecules of low superhelix density as intermediate forms in plasmid replication. Timmis K, Cabello F, Cohen SN. Nature. 1976; 261 (5560): 512-6
Replication control in a composite plasmid constructed by in vitro linkage of two distinct replicons. Cabello F, Timmis K, Cohen SN. Nature. 1976; 259 (5541): 285-90
Structural evolution of bacterial plasmids: role of translocating genetic elements and DNA sequence insertions. Cohen SN, Kopecko DJ. Fed Proc. 1976; 35 (9): 2031-6
Uniform nomenclature for bacterial plasmids: a proposal. Novick RP, Clowes RC, Cohen SN, Curtiss R, Datta N, Falkow S. Bacteriol Rev. 1976; 40 (1): 168-89
Gene manipulation. Cohen SN, N Engl J Med. 1976; 294 (16): 883-9
Replication region fragments cloned from Flac+ are identical to EcoRI fragment f5 of F. Skurray RA, Guyer MS, Timmis K, Cabello F, Cohen SN, Davidson N, Clark AJ. J Bacteriol. 1976; 127 (3): 1571-5
Involvement of multiple translocating DNA segments and recombinational hotspots in the structural evolution of bacterial plasmids. Kopecko DJ, Brevet J, Cohen SN. J Mol Biol. 1976; 108 (2): 333-60
Transposable genetic elements and plasmid evolution. Cohen SN, Nature. 1976; 263 (5580): 731-8
Detection and prevention of drug interactions utilizing an on-line computer system. Tatro DS, Briggs RL, Chavez-Pardo R, Feinberg LS, Hannigan JF, Moore TN, Cohen SN. Drug Inf J. 1975 Jan-Apr; 9 (1): 10-7
Indirect selection of bacterial plasmids lacking identifiable phenotypic properties. Kretschmer FJ, Chang AC, Cohen SN. J Bacteriol. 1975; 124 (1): 225-31
Cloning, isolation, and characterization of replication regions of complex plasmid genomes. Timmis K, Cabello F, Cohen SN. Proc Natl Acad Sci U S A. 1975; 72 (6): 2242-6
Isolation of histone genes from unfractionated sea urchin DNA by subculture cloning in E. coli. Kedes LH, Chang AC, Houseman D, Cohen SN. Nature. 1975; 255 (5509): 533-8
The manipulation of genes. Cohen SN, Sci Am. 1975; 233 (1): 25-33
alphabeta sequence of F is IS31. Hu S, Ptashne K, Cohen SN, Davidson N. J Bacteriol. 1975; 123 (2): 687-92
The organization of sea urchin histone genes. Kedes LH, Cohn RH, Lowry JC, Chang AC, Cohen SN. Cell. 1975; 6 (3): 359-69
Studies of mouse mitochondrial DNA in Escherichia coli: structure and function of the eucaryotic-procaryotic chimeric plasmids. Chang AC, Lansman RA, Clayton DA, Cohen SN. Cell. 1975; 6 (2): 231-44
Computer-based consultations in clinical therapeutics: explanation and rule acquisition capabilities of the MYCIN system. Shortliffe EH, Davis R, Axline SG, Buchanan BG, Green CC, Cohen SN. Comput Biomed Res. 1975; 8 (4): 303-20
Site specific recA--independent recombination between bacterial plasmids: involvement of palindromes at the recombinational loci. Kopecko DJ, Cohen SN. Proc Natl Acad Sci U S A. 1975; 72 (4): 1373-7
Occurrence of insertion sequence (IS) regions on plasmid deoxyribonucleic acid as direct and inverted nucleotide sequence duplications. Ptashne K, Cohen SN. J Bacteriol. 1975; 122 (2): 776-81
Online drug interaction surveillance. Tatro DS, Briggs RL, Chavez-Pardo R, Feinberg LS, Hannigan JF, Moore TN, Cohen SN. Am J Hosp Pharm. 1975; 32 (4): 417-20
Utilization of two distinct modes of replication by a hybrid plasmid constructed in vitro from separate replicons. Timmis K, Cabello F, Cohen SN. Proc Natl Acad Sci U S A. 1974; 71 (11): 4556-60
Aryl hydrocarbon hydroxylase induction in mouse peritoneal macrophages and blood-derived human macrophages. Ptashne K, Brothers L, Axline SG, Cohen SN. Proc Soc Exp Biol Med. 1974; 146 (2): 585-9
Genome construction between bacterial species in vitro: replication and expression of Staphylococcus plasmid genes in Escherichia coli. Chang AC, Cohen SN. Proc Natl Acad Sci U S A. 1974; 71 (4): 1030-4
Replication and transcription of eukaryotic DNA in Escherichia coli. Morrow JF, Cohen SN, Chang AC, Boyer HW, Goodman HM, Helling RB. Proc Natl Acad Sci U S A. 1974; 71 (5): 1743-7
Transformation of Salmonella typhimurium by plasmid deoxyribonucleic acid. Lederberg EM, Cohen SN. J Bacteriol. 1974; 119 (3): 1072-4
A method for selective cloning of eukaryotic DNA fragments in Escherichia coli by repeated transformation. Cohen SN, Chang AC. Mol Gen Genet. 1974; 134 (2): 133-41
Molecular and genetic studies of an R factor system consisting of independent transfer and drug resistance plasmids. van Embden J, Cohen SN. J Bacteriol. 1973; 116 (2): 699-709
An artificial intelligence program to advise physicians regarding antimicrobial therapy. Shortliffe EH, Axline SG, Buchanan BG, Merigan TC, Cohen SN. Comput Biomed Res. 1973; 6 (6): 544-60
Construction of biologically functional bacterial plasmids in vitro. Cohen SN, Chang AC, Boyer HW, Helling RB. Proc Natl Acad Sci U S A. 1973; 70 (11): 3240-4
Recircularization and autonomous replication of a sheared R-factor DNA segment in Escherichia coli transformants. Cohen SN, Chang AC. Proc Natl Acad Sci U S A. 1973; 70 (5): 1293-7
Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. II. Structure of drug resistance (R) factors and F factors. Sharp PA, Cohen SN, Davidson N. J Mol Biol. 1973; 75 (2): 235-55
Lack of specific inhibition of Vaccinia plaque formation by bacteriophage lambda products. Brown A, Cohen SN. Infect Immun. 1973; 7 (6): 862-4
Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Cohen SN, Chang AC, Hsu L. Proc Natl Acad Sci U S A. 1972; 69 (8): 2110-4
Further studies on the synthesis of RNA in vitro by enzyme--template complexes isolated from induced lambda lysogens. Cohen SN, Hurwitz J. J Mol Biol. 1971; 58 (2): 635-9
Non-chromosomal antibiotic resistance in bacteria. II. Molecular nature of R-factors isolated from Proteus mirabilis and Escherichia coli. Cohen SN, Miller CA. J Mol Biol. 1970; 50 (3): 671-87
Genetic expression in bacteriophage lambda. 3. Inhibition of Escherichia coli nucleic acid and protein synthesis during lambda development. Cohen SN, Chang AC. J Mol Biol. 1970; 49 (3): 557-75
Non-chromosomal antibiotic resistance in bacteria. 3. Isolation of the discrete transfer unit of the R-factor R1. Cohen SN, Miller CA. Proc Natl Acad Sci U S A. 1970; 67 (2): 510-6
Multiple molecular species of circular R-factor DNA isolated from Escherichia coli. Cohen SN, Miller CA. Nature. 1969; 224 (5226): 1273-7
Genetic transcription in bacteriophage lambda: studies of lambda m RNA synthesis in vivo. Cohen SN, Hurwitz J. J Mol Biol. 1968; 37 (3): 387-406
Role of DNA in RNA synthesis. XI. Selective transcription of gamma DNA segments in vitro by RNA polymerase of Escherichia coli. Cohen SN, Maitra U, Hurwitz J. J Mol Biol. 1967; 26 (1): 19-38
Specificity of in vitro initiation and synthesis of RNA. Maitra U, Cohen SN, Hurwitz J. Proc Can Cancer Conf. 1967; 7 113-32
Transcription of complementary strands of phage lambda-DNA in vivo and in vitro. Cohen SN, Hurwitz J. Proc Natl Acad Sci U S A. 1967; 57 (6): 1759-66
Investigations of the possible relation of ferrihemic acid to drug resistance in Plasmodium berghei. Phifer KO, Yielding KL, Cohen SN. Exp Parasitol. 1966; 19 (1): 101-9
Specificity of initiation and synthesis of RNA from DNA templates. Maitra U, Cohen SN, Hurwitz J. Cold Spring Harb Symp Quant Biol. 1966; 31 113-22
Inhibition of DNA and RNA polymerase reactions by chloroquine. Cohen SN, Yielding KL. Proc Natl Acad Sci U S A. 1965; 54 (2): 521-7
SPECTROPHOTOMETRIC STUDIES OF THE INTERACTION OF CHLOROQUINE WITH DEOXYRIBONUCLEIC ACID. COHEN SN, YIELDING KL. J Biol Chem. 1965; 240 3123-31
OBSERVATIONS ON THE HISTONE/DNA RATIO OF THE IMMATURE RABBIT UTERUS FOLLOWING ADMINISTRATION OF ESTROGEN. COHEN SN, SPICER SS, YIELDING KL. Biochim Biophys Acta. 1964; 87 511-4
COMPLEX FORMATION BETWEEN CHLOROQUINE AND FERRIHAEMIC ACID IN VITRO, AND ITS EFFECT ON THE ANTIMALARIAL ACTION OF CHLOROQUINE. COHEN SN, PHIFER KO, YIELDING KL. Nature. 1964; 202 805-6
Comparison of autologous, homologous, and heterologous normal skin grafts in the hamster cheek pouch. COHEN SN, Proc Soc Exp Biol Med. 1961; 106 677-80