Daniel DiGiulio
Academic Appointments
- Clinical Instructor, Medicine - Infectious Diseases
Key Documents
Contact Information
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Clinical Offices
Infectious Disease 300 Pasteur Dr MC 5107 Stanford, CA 94305 Tel Work (650) 723-6661 Fax (650) 498-7011
- Academic Offices
Personal Information EmailAlternate Contact EmailNot for medical emergencies or patient use
Professional Overview
Clinical Focus
- Infectious Disease
Professional Education
| Board Certification: | Infectious Disease, American Board of Internal Medicine (2005) |
| Fellowship: | Stanford University Medical Center CA (2005) |
| Residency: | Loyola University Medical Center IL (1996) |
| Internship: | St. Joseph Hospital IL (1994) |
| Medical Education: | Loyola University Chicago Stritch Sch. Of Med. IL (1992) |
| BS: | University of Illinois at Urbana-Champaign, Biology (1988) |
Graduate & Fellowship Program Affiliations
Community and International Work
- Orientation Leader, Support for International Change, Tanzania, Africa
Internet Links
Scientific Focus
Current Research Interests
My research explores the genetic diversity of human-associated microbiota in health and disease, and the accompanying host transcriptional response. This work relies largely on the application of cultivation-independent methods. An overarching goal of this research is to illuminate stereotypic patterns of microbial community assembly that are associated with specific clinical syndromes. This includes: i) profiling the commensal microbiota in distinct anatomic sites of interest; ii) identifying uncultivated pathogens associated with cryptic infections; iii) enumerating fastidious and minority constituents of polymicrobial infections; and, iv) characterizing perturbations of mixed microbial communities that confer increased disease risk. To achieve these aims, we are using various broad-range molecular approaches, including highly-parallel and quantitative methods, to characterize spatial, temporal and dose-response associations of specific microbial groups with physiologic and pathologic host states. The methodologies I use include, among others, sequence analysis of rDNA clone libraries, real-time quantitative PCR, microbial rDNA-based microarrays, and human cDNA-based microarrays.
The current thrust of my research emphasizes microbial invasion of the amniotic cavity in association with cryptic cases of preterm delivery. We are fortunate to have an ongoing collaboration with the research group of Roberto Romero, MD, Chief, Perinatology Research Branch, NICHD, NIH to investigate - in a broad and comprehensive manner - patterns of microbial prevalence, diversity and abundance that are associated with preterm delivery and its adverse neonatal sequelae. We also seek to characterize, by means of genome-scale transcriptional profiling of the host response, gene expression patterns that are predictive of preterm delivery.
Publications
- Microbial invasion of the amniotic cavity in preeclampsia as assessed by cultivation and sequence-based methods. J Perinat Med. 2010
- Microbial invasion of the amniotic cavity in pregnancies with small-for-gestational-age fetuses. J Perinat Med.. 2010
- Prevalence and Diversity of Microbes in the Amniotic Fluid, the Fetal Inflammatory Response, and Pregnancy Outcome in Women with Preterm Prelabor Rupture of Membranes. Am J Reprod Immunol. 2010; (1): 38-57
- Majority Rules? Tallying the Microbial Census in an Abscess by Means of Molecular Methods. Clin Infect Dis. 2009; (9): 1179-81
- Microbial prevalence, diversity and abundance in amniotic fluid during preterm labor: a molecular and culture-based investigation. PLoS One. 2008; (8): e3056
- Development of the human infant intestinal microbiota. PLoS Biol. 2007; (7): e177
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