Jian Cao

Publication Details

• Wavefront aberration measurements and corrections through thick tissue using fluorescent microsphere reference beacons.

  Azucena O, Crest J, Cao J, Sullivan W, Kner P, Gavel D, Dillon D, Olivier S, Kubby J. Opt Express. 2010; 18 (16): 17521-32

  We present a new method to directly measure and correct the aberrations introduced when imaging through thick biological tissue. A Shack-Hartmann wavefront sensor is used to directly measure the wavefront error induced by a Drosophila embryo. The wavefront measurements are taken by seeding the embryo with fluorescent microspheres used as "artificial guide-stars." The wavefront error is corrected in ten millisecond steps by applying the inverse to the wavefront error on a micro-electro-mechanical deformable mirror in the image path of the microscope. The results show that this new approach is capable of improving the Strehl ratio by 2 times on average and as high as 10 times when imaging through 100 microm of tissue. The results also show that the isoplanatic half-width is approximately 19 microm resulting in a corrected field of view 38 microm in diameter around the guide-star.

  PubMedID: 20721137