Francis Blankenberg
Publication Details
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Improved detection of cell death in vivo with annexin V radiolabeled by site-specific methods.
J Nucl Med. 2006; (9): 1546-53
Labeled annexin V is widely used to detect cell death in vitro and in vivo. Nearly all studies have been done with annexin V derivatized via amine-directed bifunctional agents; it was thought that these molecules retained full bioactivity compared with unmodified protein. We now show that this assumption is incorrect by measuring the affinity of annexin V for cells in vitro by quantitative calcium titration under conditions of low membrane occupancy.
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