Biophysics–

Roger Kornberg

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Publication Details

  • Yeast RNA polymerase II transcription reconstituted with purified proteins.

    Myers LC, Leuther K, Bushnell DA, Gustafsson CM, Kornberg RD. Methods. 1997; 12 (3): 212-6

    Protocols are presented for the preparation of a fully defined yeast RNA polymerase II transcription system, consisting of essentially pure TFIIB, -E, -F, and -H, TATA-binding protein, RNA polymerase II, and mediator of transcriptional regulation. This system, comprising 44 polypeptides, is able to initiate transcription at any of a dozen yeast and mammalian promoters thus far tested and responds to a variety of transcriptional activator proteins.

    PubMedID: 9237165

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