Roger Kornberg

Publication Details

• Structure of the mediator head module bound to the carboxy-terminal domain of RNA polymerase II.

  Robinson PJ, Bushnell DA, Trnka MJ, Burlingame AL, Kornberg RD. Proc Natl Acad Sci U S A. 2012; 109 (44): 17931-5

  The X-ray crystal structure of the Head module, one-third of the Mediator of transcriptional regulation, has been determined as a complex with the C-terminal domain (CTD) of RNA polymerase II. The structure reveals multiple points of interaction with an extended conformation of the CTD; it suggests a basis for regulation by phosphorylation of the CTD. Biochemical studies show a requirement for Mediator-CTD interaction for transcription.

  PubMedID: 23071300