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Roger KornbergAcademic Appointments
Appointment
Organization
Professor
Member
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Postdoctoral Advisees
Maia Azubel,
Brian Gibbons,
Craig Kaplan,
Karl-Magnus Larsson,
Xin Liu,
Kenji Murakami,
Philip Robinson,
Dong Wang
Web Site Links
Research/Lab website:
Nobel Prize Press Coverage
Research Interests
Our goal is to elucidate the fundamental basis of gene regulation. We study the control of transcription, the first step in the pathway of gene expression. Current work focuses on discovery of the molecular machines involved in transcription, reconstitution of the process with purified components, structure determination of the transcription machinery, and structure-function relationships in chromatin, the natural DNA template for transcription.
Highlights of work from the past three years include:
1. Discovery of a human homolog of the 20-protein yeast Mediator complex. Mediator is the central processing unit of gene regulation, receiving both positive and negative inputs and transducing the information to the transcription machinery.
2. Structure determination of 10-subunit, half million Dalton RNA polymerase II, in the act of transcription, with template DNA and protduct RNA, by X-ray crystallography at atomic resolution.
3. Structure determination of the entire transcription initiation complex by two-dimensional protein crystallography.
4. Discovery of a stably altered nucleosome produced by a purified chromatin-remodeling complex.
Current work is directed towards the structure of the entire transcription apparatus at atomic resolution and the mechanism of transcription control in living cells.
Highlights of work from the past three years include:
1. Discovery of a human homolog of the 20-protein yeast Mediator complex. Mediator is the central processing unit of gene regulation, receiving both positive and negative inputs and transducing the information to the transcription machinery.
2. Structure determination of 10-subunit, half million Dalton RNA polymerase II, in the act of transcription, with template DNA and protduct RNA, by X-ray crystallography at atomic resolution.
3. Structure determination of the entire transcription initiation complex by two-dimensional protein crystallography.
4. Discovery of a stably altered nucleosome produced by a purified chromatin-remodeling complex.
Current work is directed towards the structure of the entire transcription apparatus at atomic resolution and the mechanism of transcription control in living cells.
Publications
- Kornberg RD, "The molecular basis of eukaryotic transcription." Proc Natl Acad Sci U S A 2007; 104: 32: 12955-61 More »
- Kornberg R, "The Molecular Basis of Eukaryotic Transcription (Nobel Lecture)." Angew Chem Int Ed Engl 2007; More »
- Kornberg RD, Lorch Y "Chromatin rules." Nat Struct Mol Biol 2007; 14: 11: 986-8 More »
- Jadzinsky PD, Calero G, Ackerson CJ, Bushnell DA, Kornberg RD "Structure of a thiol monolayer-protected gold nanoparticle at 1.1 A resolution." Science 2007; 318: 5849: 430-3 More »
- Lorch Y, Maier-Davis B, Kornberg RD "Chromatin remodeling by nucleosome disassembly in vitro." Proc Natl Acad Sci U S A 2006; More »
137 publications: view full list
