Robert Lane Smith
Academic Appointments
- Professor (Research), Orthopaedic Surgery
- Professor (Research) (By courtesy), Mechanical Engineering
- Member, Bio-X
Professional Snapshot
Postdoctoral Advisees
Industry Relationships
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| Consulting: | Histogenics |
Scientific Focus
Research Interests
Our group is interested in the molecular and cell biology underlying bone and cartilage metabolism in health and disease. Normal daily activities are linked to the ability of the articular cartilage to withstand normal joint forces that may reach 5-7 times body weight and bone homeostasis depends on daily mechanical loading histories. The phenotypic stability of cartilage and bone depends on a complex interplay between stimuli influencing cell metabolism, physical forces, cytokines, hormones and growth factors, and the genetic expression determining the material properties of the tissue. Our lab applies modern biochemical techniques to analyze:
1. Mechanisms of cartilage degradation in inflammation and sepsis;
2. Stimulation of cartilage growth and repair by growth factors and hormones in serum-free culture;
3. Effects of adherence and deposition of glycocalyx on bacterial resistance to antibiotic treatment;
4. Effects of mechanical stresses and strains on cartilage and bone cell gene expression and matrix
syntheses;
5. Analysis of metal particles on bone resorption and prosthetic loosening in total joint arthroplasty.
The experimental techniques include development of primary cultures of human chondrocytes, quantification of proteoglycan and collagen synthesis and degradation, zymogen and kinetic analysis of neutral metalloproteinases, western analysis of protein expression, northern and slot blot analysis of mRNA levels and cloning of connective tissue and bacterial genes.
Publications
- Ultrahigh molecular weight polyethylene wear debris inhibits osteoprogenitor proliferation and differentiation in vitro. J Biomed Mater Res A. 2009; (1): 242-7
- Efficacy of a p38 mitogen activated protein kinase inhibitor in mitigating an established inflammatory reaction to polyethylene particles in vivo. J Biomed Mater Res A. 2009; (1): 117-23
- In vivo murine model of continuous intramedullary infusion of particles--a preliminary study. J Biomed Mater Res B Appl Biomater. 2009; (1): 250-3
- Biocompatibility of poly(ethylene glycol)/poly(acrylic acid) interpenetrating polymer network hydrogel particles in RAW 264.7 macrophage and MG-63 osteoblast cell lines. J Biomed Mater Res A. 2009; (3): 894-902
- Continuous intramedullary polymer particle infusion using a murine femoral explant model. J Biomed Mater Res B Appl Biomater. 2008; (2): 440-6
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