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John Boothroyd
Email:
Profile: http://med.stanford.edu/profiles/John_Boothroyd/
Alternate Contact: Academic Appointments
Appointment
Organization
Professor
Member
Member
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NIH Biosketch PDFHonors & Awards
Title
Organization
Date(s)
Leuckart Medal
German Society for Parasitology
2008
Fellow
American Academy of Microbiology
2007
Senior Scholar in Global Infectious Diseases
Ellison Medical Foundation
2002-2006
Dunlevie Family University Fellow in Undergraduate Education
Stanford University
2002
Merit Award
NIH
1994-2004
7 honors and awards: view full list
Administrative Appointments
Title
Organization
Start Year
End Year
Associate Vice-Provost for Graduate Education
Stanford University
2008
-
Senior Associate Dean for Research and Training
Stanford University School of Medicine
2002
2005
Chair, Dept. Microbiology and Immunology
Stanford University School of Medicine
1999
2002
Chair
Burroughs Wellcome Fund Advisory Panel on Molecular Parasitology
1999
2001
Chair
Gordon Conference on Parasitism
1999
1999
6 appointments: view full list
Professional Education
Degree
Awarding Institution
Field of Study
Year of Graduation
Ph.D.
Edinburgh University
Molecular Biology
1979
B.Sc. (Hons)
McGill University
Cell, Mol. and Devel. Biology
1975
Postdoctoral Advisees
Jon Boyle,
Kerry Buchholz,
Anita Koshy,
Melissa Lodoen,
Michael Reese,
Jessica Tyler,
Gusti Zeiner
Web Site Links
Research/Lab website:
Boothroyd Lab Home Page
Research Interests
Studies on the cell and molecular biology of parasitic protozoa are critically important for two reasons; first, these organisms are major pathogens of humans and anaimals and, second, they have proven to be a source of some remarkable phenomena that have challenged much of the dogma thought to be universal in eukaryotic biology. We have been studying two of these single-celled eukaryotes, Trypanosoma brucei and Toxoplasma gondii. Each has its own features that make it interesting to the scientist and both are major pathogens, trypanosomes being the cause of sleeping sickness in Africa and Toxoplasma being a major opporunistic pathogen of AIDS patients. As of, 1998, however, we have focused our entire effort on Toxoplasma because of its growing importance and our results developing this system for modern genetic analysis (we now have a full genetic "toolbox" for this intracellular parasite including a genetic map, efficient genetic transformation and gene knock-out).
The major areas where the lab is currently working are:
(i) Intracellular parasitism: how does this parasite attach, invade and reproduce within virtually any nucleated cell.
(ii) Protein trafficking; how are proteins destined for novel organelles specifically targeted.
(iii) Developmental biology; what genes are crucial for asexual development from the actively dividing to the latent form of the parasite and what are the cis- and trans-elements that control that expression.
(iv) Host-pathogen interaction: what changes occur in the host cell in response to infection?
(v) Pathogenesis: what properties make certain strains more virulent than others?
The major areas where the lab is currently working are:
(i) Intracellular parasitism: how does this parasite attach, invade and reproduce within virtually any nucleated cell.
(ii) Protein trafficking; how are proteins destined for novel organelles specifically targeted.
(iii) Developmental biology; what genes are crucial for asexual development from the actively dividing to the latent form of the parasite and what are the cis- and trans-elements that control that expression.
(iv) Host-pathogen interaction: what changes occur in the host cell in response to infection?
(v) Pathogenesis: what properties make certain strains more virulent than others?
Publications
- Boothroyd JC, Dubremetz JF "Kiss and spit: the dual roles of Toxoplasma rhoptries." Nat Rev Microbiol 2008; 6: 1: 79-88 More »
- Saeij JP, Coller S, Boyle JP, Jerome ME, White MW, Boothroyd JC "Toxoplasma co-opts host gene expression by injection of a polymorphic kinase homologue." Nature 2007; 445: 7125: 324-7 More »
- Saeij JP, Boyle JP, Coller S, Taylor S, Sibley LD, Brooke-Powell ET, Ajioka JW, Boothroyd JC "Polymorphic secreted kinases are key virulence factors in toxoplasmosis." Science 2006; 314: 5806: 1780-3 More »
- Boyle JP, Rajasekar B, Saeij JP, Ajioka JW, Berriman M, Paulsen I, Roos DS, Sibley LD, White MW, Boothroyd JC "Just one cross appears capable of dramatically altering the population biology of a eukaryotic pathogen like Toxoplasma gondii." Proc Natl Acad Sci U S A 2006; 103: 27: 10514-9 More »
- Cleary MD, Meiering CD, Jan E, Guymon R, Boothroyd JC "Biosynthetic labeling of RNA with uracil phosphoribosyltransferase allows cell-specific microarray analysis of mRNA synthesis and decay." Nat Biotechnol 2005; 23: 2: 232-7 More »
141 publications: view full list
