Daniel Bernstein

Clinical Focus

• Pediatric Cardiology
• Pediatric Heart Transplantation
• Pediatric Heart Failure

Administrative Appointments

• Chief, Division of Pediatric Cardiology, Stanford University (1994 - 2011)
• Director, Children's Heart Center, Packard Children's Hospital at Stanford (2001 - 2011)

Honors and Awards

• Alfred Woodley Salter and Mabel G. Salter Endowed Professor of Pediatrics, Stanford University (April 2004-present)

Professional Education

Postdoctoral Advisees

Chesney Castleberry,  Michael Coronado,  Viswanathan Rajagopalan

Internet Links

• Murine CV Core Lab/Bernstein Lab Site

Current Research Interests

We are interested in the role of the sympathetic nervous system and its cellular components (adrenergic receptors, G proteins) in regulating cardiac development and function. We are also interested in developing tools for examination of cardiovascular physiologic parameters in transgenic and targeted gene disruption mouse models of cardiovascular disease. Specific projects underway in our lab include:

1. Evaluation of the role of beta1 and beta2 adrenergic receptor subtypes in regulating cardiac structure and function by producing mice with targeted gene disruption of these receptors.

2. Evaluation of the role of crosstalk between beta receptors and other signaling pathways (MAPK,Akt,PKC) in regulating cardiac structure and function.

3. Role of beta receptors in regulation of intracellular and intramitochondrial calcium.

4. Role of beta receptors in adriamycin cardiotoxicity.

5. Exercise physiology in the mouse

6. Gene microarray analysis of cardiomyopathy models.

We also are interested in clinical cardiac transplantation in children, specifically:

1. The application of gene microarray methods of cardiac allograft rejection in pediatric patients.

2. Assessment of new techniques of immunosuppression in children.

3. Long-term complications in pediatric heart transplant patients.

Publications

• Dynamic microRNA Expression During the Transition from Right Ventricular Hypertrophy to Failure. Reddy S, Zhao M, Hu DQ, Fajardo GA, Hu S, Ghosh Z, Rajagopalan V, Wu JC, Bernstein D. Physiol Genomics. 2012
• In vivo functional and transcriptional profiling of bone marrow stem cells after transplantation into ischemic myocardium. Sheikh AY, Huber BC, Narsinh KH, Spin JM, van der Bogt K, de Almeida PE, Ransohoff KJ, Kraft DL, Fajardo G, Ardigo D, Ransohoff J, Bernstein D, Fischbein MP, Robbins RC, Wu JC. Arterioscler Thromb Vasc Biol. 2012; 32 (1): 92-102
• Outcomes of Children Following a First Hospitalization for Dilated Cardiomyopathy. Hollander SA, Bernstein D, Yeh J, Dao D, Sun HY, Rosenthal D. Circ Heart Fail. 2012
• Cardiac pressure overload hypertrophy is differentially regulated by β-adrenergic receptor subtypes. Zhao M, Fajardo G, Urashima T, Spin JM, Poorfarahani S, Rajagopalan V, Huynh D, Connolly A, Quertermous T, Bernstein D. Am J Physiol Heart Circ Physiol. 2011; 301 (4): H1461-70
• Clinical usefulness of a novel C1q assay to detect immunoglobulin G antibodies capable of fixing complement in sensitized pediatric heart transplant patients. Chin C, Chen G, Sequeria F, Berry G, Siehr S, Bernstein D, Rosenthal D, Reinhartz O, Tyan D. J Heart Lung Transplant. 2011; 30 (2): 158-63