Protocols
We are proud to be part of a collaborative effort to establish the small sea anemone Aiptasia as a model organism.
Efforts from the lab have led to consistent spawning in a laboratory setting and optimized husbandy techniques. View all spawning data from the Pringle Lab in this interactive web app.
All vetted protocols are published on protocols.io. Below are the most commonly requested protocols:
Pringle Lab Husbandry Protocol
Pringle Lab Spawning and Embryo Handling Protocol
Cold Shock to bleach Aiptasia Protocol
Simultaneous DNA isolation Aiptasia & Symbiodinium Protocol
Table of Antibodies Tested by the Pringle Lab
Antibody Name |
Immunogen |
Applications (IHC, WB) |
Successful? |
Notes |
Tested by |
Supplier |
Catalog Number |
anti-histone H2A |
Histone H2A (Human) |
IHC of whole mounts and sections |
Yes |
Rabbit polyclonal; good for staining Aiptasia nuclei specifically; used at 1:500 |
Santiago Perez |
Abcam |
ab58550 |
anti-Histone H3 (phospho T3) |
Histone H3 phosphorylated at T3 (S. cerevisiae) |
IHC whole mounts and sections |
Yes |
Rabbit polyclonal, Stains mitotic chromatic of Aiptasia really well, very little background; used at 1:500 in IHC |
Santiago Perez |
Abcam |
ab17352 |
anti-phospho-Histone H3 (Ser 10) |
Histone H3 phosphorylated at Ser10 (Human) |
IHC of whole mounts and sections |
Yes |
Rabbit polyclonal, Stains mitotic chromatic of Aiptasia very well with very little background; used at 1:500 in IHC |
Santiago Perez |
Millipore |
06-570 |
anti-Ki67 |
Ki67 (Human) |
IHC of sections |
No |
Rabbit monoclonal; tested to label proliferating cells; tested at 1:100; 1:500 |
Santiago Perez |
Thermo Scientific |
RM-9106 |
anti-YOL1/34 |
Tubulin (S. cerevisiae) |
IHC of whole mounts, WB |
Yes |
Rat monoclonal; stains mitotic spindle; used at 1:500; band at 51 kD in WB |
Santiago Perez, Liz Hambleton |
Abcam |
ab6161 |
anti-Actin, clone C4 |
Actin (Chicken) |
IHC of whole mounts and sections, WB of Aiptasia protein extract |
Yes |
Mouse monoclonal, band at 42 kD in WB of Aiptasia protein; used at 1:500 in IHC |
Santiago Perez, Liz Hambleton |
Millipore |
MAB1501 |
anti-coracle |
Coracle (Drosophila) |
IHC of whole mounts |
No |
Mouse monoclonal against a component of septate junctions, tested to delineate Aiptasia cell-perimeter (obtained from Dr. Richard G. Fehon of U. of Chicage, Dept. of Mol. Gen. and Cell Bio.); tested at 1:50, 1:100, 1:500 |
Santiago Perez |
Developmental Studies Hybridoma Bank |
C566.9 and C615.16 |
anti-E-Cadherin |
epithelial cadherin (Mouse) |
IHC of whole mounts |
No |
Rat monoclonal; tested as a marker of cell perimeter (usuccessfully); tested at 1:50, 1:100, 1:500 |
Santiago Perez |
Zymed (Invitrogen) |
13-1800 |
anti-LC3 |
Residues 25-121of the human Microtuble-associated protein light chain 3 (LC3) protein sequence |
WB |
Yes |
Rabbit polyclonal, band at 14 kD in WB of Aiptasia protein |
Tamaki Bieri |
Novus Biologicals |
NB100-2331 |
gp62ap |
recombinant portion of Aiptasia C3 |
IHC of whole mounts |
Yes |
Custom antibody, guinea pig polyclonal, affinity purified by Liz Hambleton; successful in whole mount IHC |
Liz Hambleton |
Cocalico Biologicals |
- |
gp64ap |
recombinant portion of Aiptasia C3 |
IHC of whole mounts |
Yes |
Custom antibody, guinea pig polyclonal, affinity purified by Liz Hambleton; successful in whole mount IHC |
Liz Hambleton |
Cocalico Biologicals |
- |
gp65ap |
recombinant portion of Aiptasia C3 |
IHC of whole mounts |
Yes |
Custom antibody, guinea pig polyclonal, affinity purified by Liz Hambleton; successful in whole mount IHC |
Liz Hambleton |
Cocalico Biologicals |
- |
anti-C3 |
internal portion of human C3 |
WB |
No |
Goat polyclonal; successful in WB of mouse serum, but not with Aiptasia protein extract |
Liz Hambleton |
Santa Cruz Biotech |
sc-14613 |