Ex Vivo Diffusion Imaging

In vivo diffusion imaging suffers from modest interpretability and an incomplete understanding of how tissue biology affects the diffusion signal. Therefore, diffusion imaging of ex vivo brains provides a valuable link between in vivo diffusion studies and histology. The absence of motion and long scan times enable the acquisition of much higher quality data than can typically be achieved in vivo. However, the shortened T2’s and decreased diffusion rates in fixed tissue are undesirable because they decrease sensitivity. We have developed and optimized several acquisition and analysis strategies for ex vivo diffusion imaging of human brains including whole brains, which only fit inside a clinical scanner and small specimens, which may be scanned on small bore animal scanners.

High Resolution Diffusion Weighted Imaging in Fixed Human Brain Using Diffusion Weighted Steady State Free Precession

McNab JA, Jbabdi S, Deoni SCL, Douaud G, Behrens TEJ, Miller KL

NeuroImage, 2009, 46(3):775-785.

 

Reduced Limbic Connections May Contraindicate Subgenual Cingulate Deep Brain Stimulation for Intractable Depression

McNab JA, Voets NL, Jenkinson N, Squier W, Miller KL, Goodwin GM, Aziz TZ

Journal of Neurosurgery, 2009, 111(4):780-4.

 

Diffusion Imaging of Whole, Post-mortem Human Brains on a Clinical 3T Scanner

Miller KL, Stagg C, Douaud G, Jbabdi S, Chance S, Esiri M, Voets NL, Jenkinson N, Aziz TZ, Jenkinson M, Smith SM, Johansen-Berg H, McNab JA

NeuroImage, 2011, 57(1):167-181.

 

Diffusion Tractography of Post-mortem Human Brains: A Comparison of Spin Echo and Steady-State Free Precession Techniques

Miller KL, McNab JA, Jbabdi S, Douaud G

NeuroImage, 2012, 59(3):2284-2297.

 

A Combined Post-mortem MRI and Quantitative Histological Study of Multiple Sclerosis Pathology

Kolasinski J, Stagg CJ, Chance SA, Deluca GC, Esiri MM, Change EH, Palace JA, McNab JA, Jenkinson M, Miller KL, Johansen-Berg H

Brain, 2012, 135(10):2938-51 .

 

The Human Connectome Project and Beyond: Initial Applications of 300mT/m Gradients

McNab JA, Edlow BL, Witzel T, Huang SY, Bhat H, Heberlein K, Feiweier T, Liu K, Keil B, Cohen-Adad J, Tisdall MD, Folkerth RD, Kinney HC, Wald LL

NeuroImage, 2013, 80:234-45.

 

Q-space Truncation and sampling diffusion spectrum imaging

Tian Q., Rokem A., Folkerth R.D., Edlow B.L., McNab J.A.

Magnetic Resonance in Medicine, 2016, doi: 10.1002/mrm.26071.

 

Fiber Orientation Measurement Using Diffusion MRI and CLARITY on the Same Human Brain Tissue.

Leuze C, Goubran M, Aswendt M, Tian Q, Hsueh B, Zeineh M, Deisseroth K, McNab JA.

Annual Meeting of the Organization for Human Brain Mapping, Geneva, Switzerland, June 2016. ***Received a Merit Abstract Award for being one of the top-rated abstracts.**

 

Evaluation of MR Contrast in Cleared Tissue.

Leuze C, Tomer R, Tian Q, Ferenczi E, Spielman D,  Zeineh M, Deisseroth K,  McNab JA.

International Society for Magnetic Resonance in Medicine Annual Scientific Meeting: Toronto, Canada, June 2015.

 

Quantification of 3D Microscopic Tissue Features in CLARITY Data for Comparison with Diffusion MRI.

Tian Q, Leuze C, Tomer R, Ferenczi E, Zeineh M, Deisseroth K, McNab JA.

International Society for Magnetic Resonance in Medicine Annual Scientific Meeting: Toronto, Canada, June 2015.

 

MRI Evaluation of the CLARITY Method for Lipid Removal From Brain Tissue

Leuze C, Tomer R, Ferenczi E, Tian Q, Zeineh M, Deisseroth K, McNab JA.

Annual Meeting of the Organization for Human Brain Mapping: Honolulu, USA, June 2015.

 

Determining the Microstructural Basis of Human MRI Using CLARITY

Leuze C, Goubran M, Ferenczi EA, Hsueh B, Plowey E, Tian Q, Tomer R, Zeineh M, Deisseroth K, McNab JA.

Annual Meeting of the Society For Neuroscience, Chicago, October 2015.