Protocols
Histology
-Tissue Microarray
Molecular Biology
-RNA
- mRNA Isolation and Purification for cDNA microarray
- Total RNA Isolation and Purification for cDNA microarray
- Total RNA isolation from Formaldehyde Fixed Paraffin-Embedded tissues (FFPE)
- DNase treatment
- RNA amplification
-DNA
- DNA extraction from the organic phase of Trizol reagent after RNA isolation
- DNA Isolation from Frozen Tissue
- DNA extraction from Formaldehyde Fixed Paraffin-embedded Tissue (FFPE)
-cDNA library construction
- mRNA 3' end library construction for Solexa sequencing for samples from FFPE (Formaldehyde Fixed Paraffin-Embedded tissues)
-cDNA Microarrays
- mRNA labeling and array hybridization
- Amplified RNA labeling and array hybridization
- Total RNA labeling and array hybridization
- CGH-Genomic DNA labeling and array hybridization
-Human Exonic Evidence-based Oligonucleotide DNA microarrays ( HEEBO)
- Amino-allyl indirect labeling and array hybridization
- Oligo Array-Based CGH-probe labeling and hybridization (Agilent arrays)
-miRNA
-Fluorescence In Situ Hybridization
-RNA probes for In Situ Hybridization
-Non-radioactive In Situ Hybridization of Paraffin Embedded Tissues
