Peer-Reviewed Research Publications

2016

53.  Hadamitzky C, Zaitseva TS, Bazalova-Carter M, Paukshto MV, Hou L, Strassberg Z, Ferguson J, Matsuura Y, Dash R, Yang PC, Kretchetov S, Vogt PM, Rockson SG, Cooke JP, Huang NF. Aligned nanofibrillar collagen scaffolds - Guiding lymphangiogenesis for treatment of acquired lymphedema. Biomaterials 102:259-67, 2016. Pubmed

52.  Kim JJ, Hou L, Huang NF. Vascularization of three-dimensional engineered tissues for regenerative medicine applications. Acta Biomater. 41:17-26, 2016. Pubmed

51.  Ogle BM, Bursac N, Domian I, Huang NF, Menasché P, Murry CE, Pruitt B, Radisic M, Wu JC, Wu SM, Zhang J, Zimmermann WH, Vunjak-Novakovic G. Distilling complexity to advance cardiac tissue engineering.  Sci Transl Med. 8: 342ps13, 2016. Pubmed

50.  Hou L, Coller J, Natu V, Hastie TJ, Huang NF. Combinatorial Extracellular Matrix Microenvironments Promote Survival and Phenotype of Human Induced Pluripotent Stem Cell-Derived Endothelial Cells in Hypoxia. Acta Biomater. S1742-706130390-7, 2016. Pubmed

49.  Giordano S, Zhao X, Xing D, Hage F, Oparil S, Cooke JP, Lee J, Nakayama KH, Huang NF, Chen Y-F. Targeted Delivery of Human iPS-ECs Overexpressing IL-8 Receptors Inhibits Neointimal and Inflammatory Responses to Vascular Injury in the Rat. Am J Physiol Heart Circ Physiol.  Epub, 2016. Pubmed

48. Nakayama KH, Surya VN, Gole M, Walker TW, Yang W, Lai ES, Ostrowski MA, Fuller GG, Dunn AR, Huang NF.  Nanoscale Patterning of Extracellular Matrix Alters Endothelial Function under Shear Stress.  Nano Lett, 16:410-9, 2016. Pubmed

47. Hou L, Kim JJ, Woo YJ, Huang NF. Stem Cell-Based Therapies to Promote Angiogenesis in Ischemic Cardiovascular Disease. Am J Physiol Heart Circ Physiol. 310:H455-65, 2016. Pubmed.

2015

46. Dash R, Kim PJ, Matsuura Y, Ikeno F, Metzler S, Huang NF, Lyons JK, Nguyen PK, Ge X, Wong Po Foo C, McConnell MV, Wu JC, Yeung AC, Harnish P, Yang PC.  Manganese-Enhanced Magnetic Resonance Imaging Enables In Vivo Confirmation of Peri-Infarct Restoration Following Stem Cell Therapy in a Porcine Ischemia-Reperfusion Model. J Am Heart Assoc,   4: pii: e002044. doi: 10.1161/JAHA.115.002044, 2015. Pubmed

45. Nakayama KH, Joshi PA, Lai ES, Gujar P, Joubert L-M, Chen B, Huang NF. Bi-layered vascular graft derived from human induced pluripotent stem cells with biomimetic structure and function. Regen Med 10:745-55, 2015. Pubmed

44. Nakayama KH, Hong G, Lee JC, Patel J, Edwards B, Zaitseva TS, Paukshto MV, Dai H, Cooke JP, Woo YJ, Huang NF. Aligned-Braided Nanofibrillar Scaffold with Endothelial Cells Enhances Arteriogenesis.  ACS Nano, 9: 6900–6908, 2015 Pubmed

43. Yuan K, Orcholski M, Panaroni C, Shuffle EM, Huang NF, Jiang X, Tian W, Vladar EK, Wang L, Nicolls MR, Wu J, de Jesus Perez VA. Activation of the Wnt/Planar Cell Polarity Pathway Is Required for Pericyte Recruitment during Pulmonary Angiogenesis. Am J Pathol 20185:69-84, 2015.  Pubmed

2014

42. Burridge PW, Metzler SA, Nakayama K, Abilez OJ, Simmons CS, Bruce MA, Matsuura Y, Kim P, Wu JC, Butte M, Huang NF, Yang PC. Multi-cellular Interactions Sustain Long-Term Contractility of Human Pluripotent Stem Cell-Derived Cardiomyocytes. Am J Trans Res, 6:724-735, 2014. Pubmed

41. Mulyasasmita W, Cai L, Dewi RE, Jha A, Ullmann SD, Luong RH, Huang NF, Heilshorn SC. Avidity-controlled hydrogels for injectable co-delivery of induced pluripotent stem cell-derived endothelial cells and growth factors. J Control Release 191:71-81, 2014 Pubmed

40. Nakayama K, Hou L, Huang NF. Role of Extracellular Matrix Signaling Cues in Modulating Cell Fate Commitment for Cardiovascular Tissue Engineering. Adv Healthc Mater 3:628-41, 2014.Pubmed

39. Ghebremariam YT, Huang NF, Kambhampati S, Volz KS, Joshi GG, Anslyn EV, Cooke JP. Characterization of a Fluorescent Probe for Imaging Nitric Oxide. J Vasc Surg, 51:68-79, 2014.Pubmed

38. Ostrowski MA, Huang NF, Walker TW, Verwijlen T, Poplawski C, Khoo AS, Cooke JP, Fuller GG, Dunn AR. Microvascular Endothelial Cells Migrate Upstream and Align Against the Shear Stress Field Created by Impinging Flow. Biophys J 106:366-374, 2014. Pubmed

37. Hong G, Lee JC, Jha A, Diao S, Nakayama KH, Hou L, Doyle TC, Robinson JT, Antaris AL, Dai H, Cooke JP, Huang NF.  Near-Infrared II Fluorescence for Imaging Hindlimb Vessel Regeneration with Dynamic Tissue Perfusion Measurement. Circ Cardiovasc Imaging, 7:517-25, 2014. Pubmed

2013

36. Huang NF, Dewi RE, Okogbaa J, Lee JC, Rufaihah AJ, Heilshorn SC, Cooke, JC. Chemotaxis of human induced pluripotent stem cell-derived endothelial cells. Am J Transl Res 5:510-520, 2013. (*co-first author). Pubmed

35. Bakr A, Pak O, Taye A, Hamada F, Hemeida R, Janssen W, Gierhardt M, Ghofrani HA, Seeger W, Grimminger F, Schermuly RT, Witzenrath M, Brandes RP, Huang N, Cooke JP, Weissmann N, Sommer N. Effects of dimethylarginine dimethylaminohydrolase-1 overexpression on the response of the pulmonary vasculature to hypoxia.  Am J Respir Cell Mol Biol. 49:491-500, 2013. Pubmed

34. Jalil RA, Huang NF*, Kim J, Herold J, Volz KS, Park TS, Lee JC, Zambidis ET, Reijo-Pera R, Cooke, JP. Human Induced Pluripotent Stem Cell-Derived Endothelial Cells Exhibit Functional Heterogeneity. Am J Transl Res 5:21-35, 2013. (*co-first author). Pubmed

33. Huang NF, Lai E, Fuller GG, Ribeiro AS, Pan S, Pruitt B, Fuller GG, Cooke JP. Spatial patterning and shear stress modulate endothelial cell morphology, gene expression, and function: implications for vascular tissue engineering. Biomaterials 34: 2928-2937, 2013. Pubmed

32. Huang NF, Okogbaa JN, Lee JC, Paukshto M, Zaitseva T, Cooke JP. The Modulation of Endothelial Cell Morphology, Function, and Survival Using Anisotropic Nanofibrillar Collagen Scaffolds. Biomaterials, 34: 4038-4047, 2013. Pubmed

31. Li J, Huang NF*, Zou J, Laurent TJ, Lee JC, Okogbaa J, Cooke JP, Ding S. Conversion of Human Fibroblasts to Functional Endothelial Cells by Defined Factors. Arterioscler Thromb Vasc Biol 33:1366-75, 2013. (*co-first author). Pubmed

2012

30. Huang NF, Okogbaa J, Babakhanyan A, Cooke JP. Bioluminescence Imaging of Stem Cell Survival for Vascular Regeneration. Theranostics 2:346-54, 2012. Pubmed

29. Hong G, Lee JC, Robinson JT, Raaz U, Xie L, Huang NF#, Cooke JP, Dai H. Multi-Functional In Vivo Vascular Imaging Using Near-Infrared II Fluorescence. Nature Medicine, 18:1841-6, 2012 (#co-corresponding author). Pubmed

28. Lai E, Huang NF*, Cooke JP, Fuller GG. Aligned nanofibrous vascular grafts modulate endothelial cell orientation and migration Regen Med 7:649-661, 2012 (*co-first author). Pubmed

27. Wong W, Huang NF, Botham CM, Sayed N, Cooke JP. Endothelial Cells Derived From Nuclear Reprogramming. Circ Res 111:1363-1375, 2012. Pubmed

Before 2012

26. Abdul Jalil R, Huang NF*, Jame S, Lee J, Nguyen HN, Byers B, De A, Okogbaa J, Rollins MD, Reijo-Pera R, Gambhir SS, Cooke JP. Endothelial Cells Derived From Human iPSCs Increase Capillary Density and Improve Perfusion in a Mouse Model of Peripheral Arterial Disease.Arterioscler Thromb Vasc Biol,31:e72-79, 2011 (*co-first author). Pubmed

25. Huang NF, Li S. Regulation of the matrix microenvironment for stem cell engineering and regenerative medicine. Annals Biomed Eng 39:1201-1214, 2011. Pubmed

24. Huang NF, Kurpinski K, Fang Q, Lee RJ, Li S. Proteomic Identification of Biomarkers of Vascular Injury.  Am J Transl Res 3:139-148, 2011. Pubmed

23. Huang NF, Patlolla B, Abilez OA, Sharma H, Beygui RE, Zarins CK, Cooke JP. A Matrix Micropatterning Platform for Cell Localization and Stem Cell Fate Determination. Acta Biomaterialia 6: 4614-4621, 2010. Pubmed

22. Huang NF, Niiyama H, Peter C, De A, Natkunam Y, Fleissner F, Li Z, Rollins MD, Wu JC, Gambhir SS, Cooke JP. Embryonic Stem Cell-Derived Endothelial Cells Engraft into the Ischemic Hindlimb and Restore Perfusion. Arterioscler Thromb Vasc Biol 30:984-91, 2010. Pubmed

21. Huang NF, Fleissner F, Sun J, Cooke JP.  Role of Nitric Oxide Signaling in Endothelial Differentiation of Embryonic Stem Cells. Stem Cells Dev 19:1617-1625, 2010. Pubmed

20. Huang NF, Chu J, Lee RJ, Li S. Biophysical and Chemical Effects of Fibrin on Mesenchymal Stromal Gene Expression.  Acta Biomaterialia 6: 3947-3956, 2010. Pubmed

19. Huang NF, Lee RJ, Li S. Engineering of Aligned Skeletal Muscle by Micropatterning. Am J Transl Res, 2:19-31, 2010. Pubmed

18. Yu J,  Huang NF*, Wilson KD, Velotta JB, Huang M, Li Z, Lee A, Robbins RC, Cooke JP, Wu JC. nAChRs Mediate Human Embryonic Stem Cell-Derived Endothelial Cells: Proliferation, Apoptosis, and Angiogenesis. PLos One, 4:e7040, 2009 (*co-first author). Pubmed

17. Huang NF, Lam A, Fang Qizhi, Sievers RE, Li S, Lee RJ. Bone Marrow-Derived Mesenchymal Stem Cells in Fibrin Augments Angiogenesis in the Chronically Infarcted Myocardium. Regen Med4:527-538, 2009. Pubmed

16. Huang NF, Niiyama H, De A, Gambhir SS, Cooke JP. Embryonic Stem Cell-Derived Endothelial Cells for Treatment of Hindlimb Ischemia. J Vis Exp 23:1034, 2009. Pubmed

15. Niiyama H, Huang NF, Rollins MD, Cooke JP. Murine model of hindlimb ischemia. J Vis Exp23:1035, 2009. Pubmed

14. Huang NF, Li S. Mesenchymal Stem Cells for Vascular Regeneration. Regen Med 3:877-92, 2008. Pubmed

13. Lee RJ, Fang Q, Davol PA, Gu Y, Sievers RE, Grabert RC, Gall JM, Tsang E, Yee MS, Fok H, Huang NF, Padbury JF, Larrick JW, Lum LG.  Antibody Targeting of Stem Cells to Infarcted Myocardium. Stem Cells 25:712-717, 2007. Pubmed

12. Park JS, Huang NF, Kurpinski KT, Patel S, Hsu S, Li S. Mesenchymal Stem Cells: Mechanobiology and cardiovascular therapies. Frontiers in Bioscience: 12:5098-5116, 2007.Pubmed

11. Huang NF, Lee RJ, Li S. Chemical and Physical Regulation of Stem Cells and Progenitor Cells: Potential for Cardiovascular Tissue Engineering. Tissue Eng: 13:1809-1823, 2007. Pubmed

10. Huang NF, Patel S, Thakar RG, Wu J, Hsiao BS, Chu B, Lee RJ, Li S.  Skeletal Muscle Morphogenesis on Micropatterned and Nanopatterned Biopolymers. Nano Letters 6:537-542, 2006. Pubmed

9. Huang NF, Sievers RE, Park JS, Fang Q, Li S, Lee RJ.  A Rodent Model of Myocardial Infarction for Testing the Efficacy of Cells and Polymers for Myocardial Reconstruction.  Nature Protocols1:1596-1609, 2006. Pubmed

8. Li S, Huang NF, Hsu S.  Mechanotransduction in Endothelial Cell Migration.  J Cell Biochem96:1110-1126, 2005. Pubmed

7. Huang NF, Yu J, Sievers R, Li S, Lee RJ.  Injectable Biopolymers Enhance Angiogenesis after Myocardial Infarction. Tissue Eng 11:1860-1866, 2005. Pubmed

6. Levenberg S, Huang NF, Lavik E, Rogers AB, Itskovitz-Eldor J, Langer R.  Differentiation of Human Embryonic Stem Cells on Three-Dimensional Polymer Scaffolds. Proc Natl Acad Sci U S A  100:12741-12746, 2003. Pubmed

5. Eisenbud D, Huang NF, Luke S, Silberklang M.  Skin Substitutes and Wound Healing: Current Status and Challenges. Wounds 16: 2-17, 2004. Article

4. Pujar NS, Huang NF, Daniels CL, Dieter L, Gayton MG, Lee AL.  Based-Catalyzed Hydrolysis of Phosphodiester Bonds in Pneumococcal Polysaccharides. Biopolymers 75: 71-84, 2004. Pubmed

3. Huang NF, Zac-Varghese S, Luke S.  Apoptosis in Skin Wound Healing. Wounds 15:182-94, 2003. Article

2. Thakar RG, Ho F, Huang NF, Liepmann D, Li S.  Regulation of Vascular Smooth Muscle Cells by Micropatterning.  Biochem Biophys Res Com  307:883-890, 2003. Pubmed

1. Huang NF, Gupta M, Varghese S, Rao S, Luke S.  Detection of Numerical Chromosomal Abnormalities in Epithelial Ovarian Neoplasms by Fluorescence in situ Hybridization (FISH) and a Review of Current Literature. Appl Immunohistochem Mol Morphol 10: 187-193, 2002. Pubmed

Textbook Chapters

5. Lee JC, Huang NF, Cooke JP. Hindlimb Ischemia. In: Manual of Research Techniques in Cardiovascular Medicine. Ed. Roberto Bolli. Wiley-Blackwell, NY, 2014 (ISBN# 0470672692)

4. Huang NF. Tissue Engineering and Regenerative Medicine: Role of Extracellular Matrix Microenvironment. In: Stem Cells and Cancer Stem Cells. Volume 9: 313-324, Springer, NY, 2013 (ISBN# 9400756445 )

3. Huang NF, Li S. Mesenchymal Stem Cells for Tissue Regeneration. In: Stem Cell and Tissue Engineering. World Scientific Publishing Co: NY, 2011 (ISBN# 9789814317054)

2. Huang NF, Gupta M, Luke S. Detection of Genetic Abnormalities in Ovarian Carcinoma. In:Immunohistochemistry and in situ Hybridization of Human Carcinomas. Vol 4, Elsevier Science Press, NY, 2006 (ISBN# 9780123339416)

1. Levenberg S, Huang NF, Itskovitz-Eldor J, Langer R.  Endothelial Cells Derived from Human Embryonic Stem Cells. In: Human ES Cells: Basic Biology and Multilineage Differentiation. Bios Scientific Publishers, Ltd., Oxford, UK, 2004. (ISBN# 9781859962787)

Patents

2012

4. Huang NF, Zaitseva T, Paukshto M, Fuller GG, Cooke JP. A Graft For Directed Vascular And
Lymphatic Regeneration And Methods To Guide Endothelial Cell Assembly.  (US Patent Provisional Application Serial #61/627,289)

3. Hong G, Lee J, Huang NF, Cooke JP, Dai H. Vascular imaging using near infrared fluorescence (PCT/2012/66157)

Before 2012

2. Su J, Huang NF. Carbon Nanotube/fiber Reinforced Three Dimensionally Ordered Nano Scale Porous Carbon and the Process. This patent describes the invention of a porous carbon device  which can be useful for electrochemical energy storage devices, thermal dispersion devices, and electric charge dispersion devices. (approved, US Patent#7252884, issued 2007)

1. Li S, Patel S, Hashi C, Huang NF, Kurpinski K. Fabrication of aligned nanofiber scaffolds for vascular and muscular tissue engineering (US Patent Application # US20070269481, filed 2007). This patent application describes the development of vascular and muscular grafts with aligned cellular and matrix structure.  Licensees: NanoVasc and NanoNerve

Published Conference Proceedings

Before 2012

6. Jalil RA, Huang NF, Jame S, Lee J, Nguyen HN, De A, Gambhir S, Reijo-Pera R, Cooke JP. Human Endothelial Cells Derived from Induced Pluripotent Stem Cells Enhance Perfusion in a Murine Model of Peripheral Arterial Disease.  Circulation Supp 122:A20680, 2010

5. Jalil RA, Nguyen HN, Huang NF, Hunter AL, Pera RR, Cooke JP. Human Induced Pluripotent Stem Cell-derived Endothelial Cells Exhibit Heterogeneity. Circulation Supp. 120:S1092, 2009

4. Huang NF, Niiyama H, Peter C, De A, Natkunam Y, Fleissner F, Li Z, Rollins MD, Wu JC, Gambhir SS, Cooke JP. Restoration of Hindlimb Perfusion by Embryonic Stem Cell-Derived Endothelial Cells. Circulation Supp 120: S1152, 2009

3. Huang NF, Lam A, Fang Q, Sievers RE, Li S, Lee RJ.  Injectable Delivery of Mesenchymal Stem Cells in Fibrin for Therapeutic Repair of Chronic Myocardial Infarction.  Circulation Supp 114:II-17, 2006

2. Huang N, Thakar R, Wong M, Kim D, Lee R, Li S. Tissue Engineering of Muscle on Micropatterned Polymer Films.  Conf Proc IEEE Eng Med Biol Soc 7:4966-4969, 2004. Pubmed

1. Luke S, Huang NF, Goyal N, Varkey JA, Alsay-Oculam AA, Gupta M, Ladoulis CT.  Optimal Fluorescence in situ Hybridization (FISH) in Cytologic Specimens. Cell Vis 5: 85-86, 1998. Pubmed

Scientific Conference Posters/Presentations

2014

Huang NF, Lai E, Zaitseva TS, Paukshto MV, Cooke JP. Collagen Nanopatterning Modulates Endothelial Cell Atheroprotective Function, Survival, and Angiogenesis. Basic Cardiovascular Sciences Scientific Sessions, July 14-17, 2014, Las Vegas, NV.

Huang NF, Okogbaa J, Jha A, Lee JC, Zaitseva T, Paukshto MV. Nanopatterned Collagen Scaffolds Promote Blood Perfusion in the Ischemic Limb. Arteriosclerosis Thrombosis and Vascular Biology Meeting Scientific Sessions, May 1-3, 2014, Toronto, Canada. (*winner of Peripheral Vascular Disease Travel Award)

Hou L, Huang NF. Extracellular Matrix-Mediated Differentiation of Human Induced Pluripotent Stem Cells. Experimental Biology, San Diego, CA, April 26-30, 2014

Nakayama K, Rando TA, Huang NF.  Engineering Vascularized Skeletal Muscle with Physiologically-Relevant Cellular Organization. 3rd Annual Symposium on Regenerative Rehabilitation. April 10-11, San Francisco, CA

Hou L, Huang NF. Extracellular matrix enhances generation of human induced pluripotent stem cell-derived endothelial cells for rehabilitative tissue engineering. 3rd Annual Symposium on Regenerative Rehabilitation. April 10-11, San Francisco, CA

Huang NF, Okogbaa J, Jha A, Zaitseva T, Paukshto MV, Cooke JP. Anisotropic Nanofibrillar Collagen Modulates Endothelial Cell Morphology, Function, and Survival. ASME Global Congress on Nanoengineering for Medicine and Biology. February 2-5, 2014, San Francisco, CA2013

2013

Huang NF, Lai E Ribeiro A, Fuller GG, Pruitt B, Cooke JP. Collagen Topographical Patterning Modulates Endothelial Cell Morphology, Gene Expression, And Function. Materials Research Society Spring Conference, April 1-5, 2013, San Francisco, CA

Ostrowski M, Huang EY, Huang NF, Walker TW, Cooke JP, Dun AR, Fuller GG. Upstream migration of endothelial cells in response to impinging fluid flows. Society of Rheology, Quebec, Canada, October 13-17, 2013

Dash R, Kim PJ, Matsuura Y, Ge X, Ikeno F, Lyons JK, Huang NF, Metzler S, Nguyen P, Heidary S, Parent M, Yamamoto T, Cooke J, Ruiz-Lozano P, Robbins RC, Wu JC, McConnell MV, Yeung A, Harnish P, Yang PC. Manganese-Enhanced cardiac MRI (MEMRI) tracks long-term in vivo survival and restorative benefit of transplanted human Amnion-Derived Mesenchymal Stem Cells (hAMSC) after porcine ischemia-reperfusion injury. 16th Annual SCMR Scientific Sessions, February 1-4, 2013, San Francisco, CA

Dash R, Kim PJ, Matsuura Y, Ge X, Ikeno F, Lyons JK, Huang NF, Metzler S, Nguyen P, Heidary S, Parent M, Yamamoto T, Cooke J, Ruiz-Lozano P, Robbins RC, Wu JC, McConnell MV, Yeung A, Harnish P, Yang PC. Sustained Restoration of LV Function in a Porcine Ischemia-Reperfusion Injury Model Using Human Placental Mesenchymal Stem Cells and Manganese-Enhanced MRI.American College of Cardiology, San Francisco, CA, March 9-11, 2013

2012

Huang NF, Ribeiro A, Lai E, Fuller GG, Pruitt B, Cooke JP. Collagen Topographical Patterning Modulates Endothelial Cell Morphology, Gene Expression, And Function. Arteriosclerosis Thrombosis and Vascular Biology Meeting. AHA Scientific Sessions, Chicago, IL, April 18-20, 2012. (*Ngan Huang was winner of Robert W Hobson II Early Career Investigator Award)

Ostrowski MA, Huang NF, Walker T, Khoo S, Devicha M, Poplawski C, Cooke JP, Fuller GG, Dunn AR. Endothelial Cell Response to Complex Flow Profiles. American Society for Cell Biology, San Francisco, December 15-19, 2012