Illumina Services

Illumina Library Preparation

Base library prices are listed here.  There are discounts for bulk submissions (more samples = greater discount), which can be seen by selecting the appropriate tab that corresponds with your number of samples.  Prices listed are for the library preparation only, sequencing is a separate charge as outlined in the sequencing section.

Library Type Price per Sample 
TruSeq Stranded mRNA $108.00
TruSeq Stranded Total RNA $168.00
Trio RNA-Seq $167.00
Universal Plus mRNA-Seq $92.00
Ovation SoLo RNA-Seq $128.00
Library Type Price per Sample
10X Single Cell 3' v3.1 $1,878.00
10X Single Cell ATAC v1.1 $1,836.00
10X Single Cell Immune Profiling V(D)J  $2,245.00

Quality Control Services

Service Type # of Samples Price 
Fragment Analyzer 1-12 $16.00
Qubit ds DNA  1-12 $14.00
Kapa Quant qPCR 1 $18.00

Illumina Sequencing

Platform Run Type Price Per Lane Average Read Output
iSeq 100 2x150 bp $926 4 Million Reads
MiSeq Micro 2x150 bp $926 4 Million Reads
 MiSeq 2x75 bp $1,496 22-25 Million Reads
2x150 bp $1,641 12-15 Million Reads
2x250 bp $1,791 12-15 Million Reads
2x300 bp $2,156 22-25 Million Reads
HiSeq 4000 2x100 bp $2,486 250-400 Million Reads
2x150 bp $2,806 250-400 Million Reads
NovaSeq 6000 SP 2x150 bp* $3,032 325-400 Million Reads
SP 2x250 bp* $3,932 325-400 Million Reads
S1 2x100 bp* $4,077 750-800 Million Reads
S1 2x150 bp* $4,677 750-800 Million Reads
S2 2x100 bp $8,500 1650-2050 Million Reads
S2 2x150 bp $9,480 1650-2050 Million Reads
S4 2x100 bp $8,186 2000-2500 Million Reads
S4 2x150 bp $9,286 2000-2500 Million Reads
Sequel Diffusion 4 SMRT cells $1,436 Inquire with Staff
8 SMRT cells $1,331 Inquire with Staff

Please visit our PacBio sequencing page to determine the loading type that is best suited for your experiment.

* These runs are very uncommonly submitted.  If you wish to run them, you should submit enough lanes to fill a full flow cell (8 lanes or multiples of 8 for multiple flow cells for HiSeq 4000 submissions, 4 lanes or multiples of 4 for S4 NovaSeq 6000 submissions, and 2 lanes or multiples of 2 lanes for S1/S2 NovaSeq 6000 submissions).  Submissions that are not in appropraite multiples are unlikely to have all of the lanes sequenced in a timely manner. A full list of all possible sequencing variants/setups is available upon request.

Other Services

Service Type

Price
NEBNext Ultra II Directional mRNA* See Notes*
NEBNext Ultra II Directional Total RNA* See Notes*
Nextera DNA Flex* See Notes*
Nextera XT WGS Metagenomic*
See Notes*
TruSeq DNA Methylation* See Notes*
Fragment Analyzer DNA* See Notes*
Fragment Analyzer RNA* See Notes*
Qubit dsDNA BR Assay* See Notes*
Qubit RNA HS Assay See Notes*

* Note: These services are not provided in Fiscal year of 2020. However, If you are interested in these services please contact the Library prep team at  gssc-libraries-submission@lists.stanford.edu.