Protocols frequently used in the Galli Lab

• Generation of bone marrow derived-cultured mast cells (BMCMC) from mouse bone marrow

• WEHI-3 cell culture and generation of conditioned medium for BMCMC culture

• BMCMC degranulation assay (ß-hexosaminidase release)

• Transient Transfection of 293T Cells for Lentiviral Production

• Engraftment of mast cell-deficient mice with bone marrow derived-cultured mast cells (BMCMC)

• Passive Cutaneous Anaphylaxis (PCA)

• Passive Systemic anaphylaxis

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