HTGTS: "Fishing" for Junctions
The high-throughput genome-wide translocation sequencing (HTGTS) platform, as a core function identifies recurrent “prey” double-stranded DNA breaks (DSBs) via their translocation to a “bait” DSB.
We leverage this junction mapping platform in a number of different contexts to gain a better understanding of nuclear processes.
Our functional genomics projects cover many different fields: radiation and cancer biology, genome editing, immunology, and cell cycle phase restricted DSB repair.
Key HTGTS feature: Readily detectable frequent DSBs
From on-target (red) and off-target (blue/green) Cas9:gRNA endonuclease bait DSBs (Frock et al., 2015). Nearly any recurrent DSB site can be used.
Modulating DSB Repair
Modulating expression of DSB repair genes provide further insight into mechanisms of genome instability
Wide-spread translocations
Widespread DSBs make the chromosome harboring the bait DSB a hotspot for translocation
Next-level Screens
Cancer cells are incentivized to modulate their DNA repair activities in order to adapt and survive. We are interested in unraveling these dependencies.
