Label-assisted de novo peptide sequencing (LADS)
People: Arun Devabhaktuni, Sam Pearlman, Sarah Lin

Computational tools that search databases of known proteins for the peptides that best match observed mass spectra make modern proteomics possible. However, for some applications where the underlying source proteome is either unknown or unwieldy, a better option is to identify peptide sequences directly from observed spectra, i.e., de novo. We have developed a computational strategy known as LADS that lets us discover peptides in this way, and are applying it to the discovery of antigenic MHC-presented peptides, and to the gut microbiome.


People: Arun Devabhaktuni, Sam Pearlman, Sarah Lin

TagGraph is a sensitive, protease independent, blind modification search tool for large and small sequence databases.