Workshop in Biostatistics

Medical School Office Building (MSOB)
Rm x303

DATE: January 18, 2018
TIME: 1:30 - 2:50 pm
TITLE: A Multiomics Clock of Human Pregnancy
Nima Aghaeepour
Faculty Member, Department of Anesthesiology, Perioperative and Pain Medicine, Stanford



The maintenance of pregnancy relies on a finely-tuned immune balance between tolerance to the fetal allograft and protective mechanisms against invading pathogens. Demonstrating the chronology of immune adaptations to a term pregnancy provides the framework for future studies examining deviations implicated in pregnancy-related pathologies including preterm birth and preeclampsia. In the first part of this presentation presentation, we demonstrate that these adaptations are precisely timed, reflecting an immune clock of pregnancy in women delivering at term. Using cytometry by time-of-flight mass spectrometry (a.k.a. CyTOF), the abundance and functional responses of billions of cells from all major immune cell subsets were quantified in serial blood samples collected throughout pregnancy. A customized Elastic-Net model, with prior distributions extracted from literature-based knowledge of the immune system, was used to develop a predictive model of inter-related immune events that accurately captured the chronology of pregnancy. In the second part, we integrate the immunological dataset with other omics datasets simultaneously collected at each time point, including data from the transcriptome, microbiome, proteome, and metabolome. We present a novel computational approach combining all available omics datasets into a predictive model that reveals unique interactions between the different pacemakers of pregnancy.

Suggested readings:

Anke Diemert, Petra Clara Arck.  Pregnancy Around the Clock.  Trends in Molecular Medicine, Vol. 24, Issue 1, p1-3, January 2018.

Nima Aghaeepour, Edward A Ganio, David Mcilwain, et. al.  An Immune clock of human pregnancy.  Science Immunology 01 Sep 2017: Vol. 2, Issue 15, eaan2946.