M112 Alway Building, Medical Center
(next to the Dean's courtyard)
|DATE:||April 27, 2017|
|TIME:||1:30 - 2:50 pm|
|TITLE:||Identify monolayer-integrated proteins from a proteomic dataset by Dirichlet mixture models|
Monolayer-integrated proteins (MIPs) could exist in any membrane of the cell, but available bioinformatics tools only identify transmembrane protein domains. To better understand the types of proteins that embed into a single leaflet of biological membranes we identified the lipid droplet (LD) monolayer-integrated proteome (MIP-ome). LDs are cytoplasmic organelles that have a reservoir of neutral lipids surrounded by a phospholipid monolayer membrane. Any protein embedded into the surface of the LD would be a MIP. Here we describe an approach that combines cutting edge proteomics with statistical modeling to identify a comprehensive set of LD-associated MIPs.
Kory, N., Farese, R.V., Walther, T.C. Targeting Fat: Mechanisms of Protein Localization to Lipid Droplets. Trends in Cell Biology, Vol. 26, Issue 7, p535-546, July 2016.