2015 Manuscript Awards
Contractility of single cardiomyocytes differentiated from pluripotent stem cells depends on physiological shape and substrate stiffness
Alexandre J. S. Ribeiro, Yen-Sin Ang, Ji-Dong Fu, Renee N. Rivas, Tamer M. A. Mohamed, Gadryn C. Higgs, Deepak Srivastava, and Beth L. Pruitt
Published in Proc Natl Acad Sci U S A. 2015 112(41)
In this study, we enhanced the maturity of myofibril organization in stem cell-differentiated cardiomyocytes with microfabrication approaches and investigated the mechanisms that drive structural maturation.
Epicardial FSTL1 reconstitution Regenerates the Adult Mammalian Heart
Ke Wei, Vahid Serpooshan, Cecilia Hurtado, Marta Diez-Cunado, Mingming Zhao, Sonomi Maruyama, Wenhong Zhu, Giovanni Fajardo, Michela Noseda, Kazuto Nakamura4, Xueying Tian, Qiaozhen Liu, AndrewWang,Yuka Matsuura, Paul Bushway, Wenqing Cai, Alex Savchenko, Morteza Mahmoudi, Michael D. Schneider, Maurice J. B. van den Hoff, Manish J. Butte, Phillip C. Yang, Kenneth Walsh, Bin Zhou, Daniel Bernstein, Mark Mercola & Pilar Ruiz-Lozano
Published in Nature 525, 479–485
Here we found that epicardial cells contain a potent cardiogenic activity identified as follistatin-like 1 (Fstl1). Application of the human Fstl1 protein via an epicardial patch stimulates cell cycle entry and division of pre-existing cardiomyocytes, improving cardiac function and survival in mouse and swine models of myocardial infarction.
Epigenetic Regulation of Phosphodiesterases 2A and 3A Underlies Compromised β Adrenergic Signaling in an iPSC Model of Dilated Cardiomyopathy
Haodi Wu, Jaecheol Lee, Ludovic G. Vincent, Qingtong Wang, Mingxia Gu, Feng Lan, Jared M. Churko, Karim I. Sallam, Elena Matsa, Arun Sharma, Joseph D. Gold, Adam J. Engler, Yang K. Xiang, Donald M. Bers, and Joseph C. Wu
Published in Cell Stem Cell: 17, 89–100
This is the first study that systematically characterized β-adrenergic signaling maturation in iPSC-CMs, which has set a baseline for all the studies focusing on beta signaling mechanism and beta-blocker drug screening in iPSC-CM models. In this paper, we have identified the up-regulation of key molecular events during the pathogenesis of familial DCM, subtype-specific blockade.
The Prognostic Value of Residual Coronary Stenoses After Functionally Complete Revascularization
Yuhei Kobayashi, Chang-Wook Nam, Pim A.L. Tonino, Takumi Kimura, Bernard De Bruyne, Nico H.J. Pijls, William F. Fearon, on behalf of the FAME Study Investigators
Published in J Am Coll Cardiol. 2016;67(14)
In this paper, we investigated the prognostic value of the residual angiographic coronary artery stenoses after FFR-guided "functionally" complete revascularization. We found that after "functionally" complete revascularization with FFR guidance, the residual angiographic lesions do not reflect residual ischemia or predict a worse outcome.