Comparative Medicine

ultrasound of pig intestine
Stephen A. Felt, DVM, MPH, Diplomate ACVPM, Diplomate ACLAM, is an assistant professor in the Department of Comparative Medicine at Stanford University School of Medicine. He earned his veterinary degree from Univeristy of Wisconsin School of Veterinary Medicine and completed his public health education at the Uniformed Services University, Bethesda, Maryland. He conducted his residency training in laboratory animal medicine at the Walter Reed Army Institue of Research (WRAIR). Dr. Felt's research interest focuses on infectious diseases, particularly zoonoses, and refining experimental techniques to improve animal health and welfare. Currently he is collaborating with labs which are studying the virulence factors of vibrio cholerae and the use of "microbubbles" for molecular imaging and as a delivery system for targeted therapies which may be translated into human and veterinary clinical applications.

Personnel

Stephen, Felt, DVM, MPH, DACLAM, DACVPM
Assistant Professor
(650) 735-5305
(650) 725-0940 (fax)

microscopic image
Heterogeneous expression of tcpA-gfp(ASV) in rabbit ileal loops and during in vitro conditions of growth that induce the expression of V. cholerae virulence genes.
(A–B) Scanning confocal fluorescence microscopy was used to visualize V. cholerae harboring the tcpA-gfp(ASV) transcriptional reporter 12 hours post inoculation of ligated ileal loops. (A) The actin-rich epithelial surfaces were stained with phalloidin (colored blue); all V. cholerae were visualized using a V. cholerae O1-specific antibody (red); bacteria expressing tcpA-gfp(ASV) (green) are shown as a composite image in (A) and in isolation in (B). Arrows indicate examples of adjacent bacteria near the epithelial surface that exhibit different levels of tcpA-gfp(ASV) fluorescence. (C) Heterogeneity of tcpA-gfp(ASV) expression after growth of the reporter strain in AKI medium. (D) Heterogeneity of tcpA-gfp(ASV) expression during early stationary phase in LB medium containing 100 mM NaHCO3. Scale bars corresponds to 15 µm. Plos Pathog 6(9), 1-23, 2010


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