Guidelines for Anesthesia, Analgesia, and Tranquilization in Amphibians
Immersion Anesthetics
- Tricaine methane sulfonate (MS222), 300-500 mg/L (larva); 500-2000 mg/L) (adults) (buffer with 10-25 m Eq/L sodium bicarbonate) (17, 19)
Injectable Anesthetics
- Tricaine methane sulfonate, 100 mg/kg; SQ; IP; SQ
(buffer with 10-25 m) Eq/L sodium bicarbonate) (17, 19)
- Tricaine methane sulfonate, 50-150 mg/kg; SQ; IM
(buffer with 10-25 m) Eq/L sodium bicarbonate) (19)
- Ketamine, 50-150 mg/kg; IM (17)
Inhalant Anesthetics - Induction
- Isoflurane, 4-5% via mask (17)
Topical Anesthetics
- Isoflurane, 0.008 to 0.06 mL/g via patch applied to skin (20)
