Abstract
DATE: |
October 15, 2009 |
TIME: |
1:15 - 3:00 pm |
LOCATION: |
Center for Clinical Sciences Research (CCSR), Rm 4205 |
TITLE: |
Robust Optimization of a Biological Protocol |
SPEAKER: |
Patrick Flaherty |
When conducting high-throughput biological experiments, a standard
protocol is often repeated many times on different samples. Such standard
protocols are usually optimized by adjusting one factor at a time until
sufficient reliability is achieved.
But this approach is both inefficient and error-prone because it can take
a considerable amount of time and effort to arrive at an acceptable design
if one is achievable at all. Furthermore, the resulting protocol is not
guaranteed to be robust to component or experimental variations. We show
how common biological protocols may be improved using an experimentally
derived model and robust optimization methods and demonstrate the
methodology on a typical polymerase chain reaction protocol.
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