FLUORESCENCE ACTIVATED CELL SORTING (FACS) FACILITY

About FACS

Since the opening of the Beckman Center in 1989 the FACS facility has provided cell analysis and sorting capabilities to Beckman Center researchers, other Stanford research groups, and to the regional biotechnology community. The facility also acts as a hub for general FACS education and provides training for users who desire to become self-operators of the facility instruments.

The FACS facility director and staff members provide, as a group, over 100 years of flow cytometry knowledge and are available to assist users in designing experiments and in data analysis. Staff members maintain the facility’s instruments and support facility operations in addition to developing improved technology for advanced applications and instrumentation.

Services

Cell Sorting

BSL-1 & BSL-2 samples, aseptic and single cell sorting into 96-well plates (cloning), and measurement and sorting using up to 18 simultaneous fluorochromes that cover almost all available fluorescent dyes. Five sorters are available, each with different capabilities.

Federated Sites

Some research laboratories, because of the nature of their work, need to have flow cytometry equipment on-site. The facility offers individualized contracts to provide management and technical consulting for these groups.

Cell Analysis

BSL-1 & BSL-2 samples, measurements of up to 18 simultaneous fluorochromes that cover almost all available fluorescent dyes, and sample runs from tubes or 96-well plates. Four analyzers are available, each with different capabilities.

Software Support

The Facility manages a site license for FlowJo data analysis software (Flowjo, Inc., Ashland, OR). The facility also licenses Cytobank (Cytobank, Inc.), a web based analysis program.

Instrument Training

Intensive instrument training is provided to users of the Facility and is tailored to the user’s needs and experimental goals.

Data Management Services

Data collected in the facility is stored and archived in a secure, highly redundant system, and made available over the web.

Mass Cytometry

Analysis of samples using Time Of Flight mass spectrometry measuring up to 100 different parameters with proteins conjugated to metal ions. 

Consulting

The expertise of the staff is available to aid researchers in experimental design and data analysis.


Director

Lisa Nichols