Cell Sciences Imaging Facility (CSIF)

About CSIF

The Cell Sciences Imaging Facility (CSIF) provides high resolution, state-of-the-art technologies for imaging and analyzing the molecular and structural organization of cells and tissue as well as bioengineered materials.  The facility offers sophisticated and demanding microscopy techniques to Stanford University and industry researchers.  The CSIF is organized into three interdependent imaging labs:  the Fluorescence Microscopy Core (FMC) which houses multi-photon, confocal, super-resolution, fluorescence lifetime and deconvolution microscopes and image analysis software; the Electron Microscopy Core (EMC), which houses high resolution scanning and transmission electron microscopes and the Array Tomography Core (ATC) that provides complete array tomography services.  The CSIF is a participating member of the Stanford Cancer Institute supporting cancer research and a designated Cancer Center facility.

In 2014, in a collaborative effort with Stanford’s School of Engineering (SOE), the CSIF opened a satellite light microscopy facility in the Shriram Center at the SOE. This new facility brings much needed biological imaging instrumentation and expertise to the Departments of Bioengineering and Chemical Engineering.


Light Microscopy

  • Confocal microscopy (scanning and spinning-disk)

  • Two-photon microscopy

  • Wide-field fluorescence microscopy

  • Digital deconvolution

  • Transmitted-light imaging (phase, DIC, histology)

  • High-content screening (Confocal and Wide-field)

  • Super-resolution imaging (STORM, STED, SIM and AiryScan)

  • Cell surface imaging with <100 nM z-resolution (TIRF)

  • Specialized microscopy (FRAP, FRET, FCS, FLIP...)

  • Fluorescence lifetime imaging microscopy (FLIM)

  • Image Analysis expertise

  • Software for image analysis, processing, and deconvolution

  • Imaris, Volocity, SVI Huygens, SoftWoRx. Microvolution

Electron Microscopy

Transmission Electron Microscopy (biological)

  • Chemical and cryo-fixation processing

  • immuno-EM

  • Correlative LM to EM: CELM

  • negative staining

  • ultramicrotomy, cryo and plastic

  • electron tomography (plastic sections)


Scanning Electron Microscopy

  • variable pressure/environmental

  • high resolution FE-SEM

  • Immuno-SEM

  • array tomography SEM, CLEM


Jon Mulholland