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Processing involves a series of tissue dehydration, clearing, and paraffin infiltration steps.
Processing prepares tissue to paraffin embedding.
Also known as blocking. This service includes embedding previously-processed tissues into paraffin (wax) blocks. Paraffin blocks can be sectioned with a microtome for making slides.
Recuts & Step Sections
RECUTS are sections made from paraffin blocks that have been previously sectioned.
STEP SECTIONS are recuts that are being obtained at a certain distance from the face of the block, and from each other (also referred to as “levels” or “measured levels”).
H & E Staining
Hematoxylin and eosin (H&E) staining is the principal stain used in histology, and regarded as the “gold standard” for brightfield microscopy.
H&E-stained slides provide a context in which to interpret specialized techniques such as special stains and immunostains.
Unstained slides can be used for further routine, special, or immunostaining.
Unstained slides can be requested as serial slides or step sections.
Immunohistochemistry (IHC) assays are based on the principle of antigen-antibody binding.
Immunohistochemistry is used to identify specific antigens and antigenic determinants (epitopes) in tissue sections.
Histochemical (special) stains aid in the visualization &/or identification of structures &/ or substances in tissue sections.
Examples: lipid, intercalated discs in cardiac cells, metals and other cell accumulations, collagen, infectious elements, basement membranes, etc.
Optimal Cutting Temperature (OCT) Medium
OCT Frozen Tissue Embedding & Sectioning
The slides resulting from OCT blocks can be used for a variety of purposes including routine, special, and immunostaining.
Updated June 8, 2020
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